Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Key Laboratory of Marine Bio-resources Sustainable Utilization (CAS), RNAM Center for Marine Microbiology (CAS), Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.
Int J Syst Evol Microbiol. 2013 Mar;63(Pt 3):1045-1050. doi: 10.1099/ijs.0.041889-0. Epub 2012 Jun 15.
A marine bacterium, designated SCSIO 03483(T), was isolated from a marine sediment sample collected from the Nansha Islands in the South China Sea. The strain produced roundish colonies with diffusible yellow-coloured pigment on nutrient agar medium or marine agar 2216. Optimal growth occurred in the presence of 0-4 % (w/v) NaCl, at pH 7.0 and a temperature range of 28-37 °C. 16S rRNA gene sequence analysis indicated that the isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarity with Imtechella halotolerans K1(T) (92.7 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the isolate shared a lineage with members of the genera Imtechella, Joostella and Zhouia. Phospholipids were phosphatidylethanolamine, two unidentified aminolipids and three unknown polar lipids. The major respiratory quinone was MK-6 and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω6c/C16 : 1ω7c). The DNA G+C content of strain SCSIO 03483(T) was 38.4 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain SCSIO 03483(T) represents a novel species in a new genus in the family Flavobacteriaceae, for which the name Sinomicrobium oceani gen. nov., sp. nov. is proposed. The type strain of Sinobacterium oceani is SCSIO 03483(T) ( = KCTC 23994(T) = CGMCC 1.12145(T)).
一株海洋细菌，命名为 SCSIO 03483(T)，从中国南海南沙群岛的海洋沉积物样本中分离得到。该菌株在营养琼脂培养基或海洋琼脂 2216 上产生圆形菌落，并有扩散性黄色色素。最佳生长条件为 0-4%（w/v）NaCl、pH 7.0 和 28-37°C。16S rRNA 基因序列分析表明，该分离株属于黄杆菌科，与耐盐伊姆泰克拉氏菌 K1(T)（92.7%）具有相对较高的序列相似性。基于近全长 16S rRNA 基因序列的系统发育分析表明，该分离株与伊姆泰克拉氏菌属、乔斯特氏菌属和周氏菌属的成员具有共同的谱系。磷脂为磷脂酰乙醇胺、两种未鉴定的氨基脂和三种未知极性脂。主要呼吸醌为 MK-6，主要脂肪酸为 iso-C15:0、iso-C17:0 3-OH 和总和特征 3（C16:1ω6c/C16:1ω7c）。菌株 SCSIO 03483(T)的 DNA G+C 含量为 38.4 mol%。根据表型、化学分类和分子数据，菌株 SCSIO 03483(T)代表黄杆菌科一个新属的新种，因此建议将其命名为海洋新微菌属 Sinomicrobium oceani gen. nov.，sp. nov.。海洋新微菌的模式菌株为 SCSIO 03483(T)（ = KCTC 23994(T) = CGMCC 1.12145(T)）。
