Li Jie, Zhang Ji, Zhao Yan-Li, Huang Heng-Yu, Wang Yuan-Zhong
Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, China.
College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming, China.
Front Chem. 2017 Dec 22;5:125. doi: 10.3389/fchem.2017.00125. eCollection 2017.
Roots, stems, leaves, and flowers of Longdan ( Franch. ex Hemsl) were collected from six geographic origins of Yunnan Province ( = 240) to implement the quality assessment based on contents of gentiopicroside, loganic acid, sweroside and swertiamarin and chemical profile using HPLC-DAD and FTIR method combined with principal component analysis (PCA). The content of gentiopicroside (major iridoid glycoside) was the highest in , regardless of tissue and geographic origin. The level of swertiamarin was the lowest, even unable to be detected in samples from Kunming and Qujing. Significant correlations ( < 0.05) between gentiopicroside, loganic acid, sweroside, and swertiamarin were found at inter- or intra-tissues, which were highly depended on geographic origins, indicating the influence of environmental conditions on the conversion and transport of secondary metabolites in . Furthermore, samples were reasonably classified as three clusters along large producing areas where have similar climate conditions, characterized by carbohydrates, phenols, benzoates, terpenoids, aliphatic alcohols, aromatic hydrocarbons, and so forth. The present work provided global information on the chemical profile and contents of major iridoid glycosides in originated from six different origins, which is helpful for controlling quality of herbal medicines systematically.
从云南省六个地理来源（共240份）采集了龙胆（Franch. ex Hemsl）的根、茎、叶和花，基于龙胆苦苷、马钱子酸、獐牙菜苷和獐牙菜苦苷的含量以及使用HPLC-DAD和FTIR方法结合主成分分析（PCA）的化学图谱进行质量评估。无论组织和地理来源如何，龙胆苦苷（主要的环烯醚萜苷）的含量在[具体来源未提及]中最高。獐牙菜苦苷的含量最低，在昆明和曲靖的样品中甚至无法检测到。在组织间或组织内发现龙胆苦苷、马钱子酸、獐牙菜苷和獐牙菜苦苷之间存在显著相关性（<0.05），这高度依赖于地理来源，表明环境条件对龙胆中次生代谢产物的转化和运输有影响。此外，样品沿着气候条件相似的大产区被合理地分为三类，其特征为碳水化合物、酚类、苯甲酸盐、萜类、脂肪醇、芳烃等。本研究提供了来自六个不同来源的龙胆的化学图谱和主要环烯醚萜苷含量的全面信息，这有助于系统地控制草药质量。
