Gharsa H, Slama K Ben, Gómez-Sanz E, Gómez P, Klibi N, Zarazaga M, Boudabous A, Torres C
Laboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université Tunis-El Manar, Tunisia.
Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.
Equine Vet J. 2015 Jul;47(4):463-6. doi: 10.1111/evj.12305. Epub 2014 Aug 14.
Staphylococcus intermedius group (SIG) bacteria can colonise the nares of some animals but are also emerging pathogens in humans and animals.
To analyse SIG nasal carriage in healthy donkeys destined for food consumption in Tunisia and to characterise recovered isolates.
Nasal swabs from 100 healthy donkeys were tested for SIG recovery, and isolates were identified by biochemical and molecular methods. Antimicrobial susceptibility of isolates was tested and detection of antimicrobial resistance and virulence genes was performed. Isolates were typed at the clonal level by multilocus sequence typing and SmaI pulsed-field gel electrophoresis.
Staphylococcus delphini and Staphylococcus pseudintermedius (included in SIG) were obtained in 19% and 2% of the tested samples, respectively, and one isolate per sample was characterised. All isolates were meticillin susceptible and mecA negative. Most S. delphini and S. pseudintermedius isolates showed susceptibility to all antimicrobials tested, with the exception of 2 isolates resistant to tetracycline (tet(M) gene) or fusidic acid. The following toxin genes were identified (percentage of isolates): lukS-I (100%), lukF-I (9.5%), siet (100%), se-int (90%), seccanine (19%) and expA (9.5%). Thirteen different pulsed-field gel electrophoresis profiles were identified among the 21 SIG isolates. Additionally, the following 9 different sequence types (STs) were detected by multilocus sequence typing, 6 of them new: ST219 (6 isolates), ST12 (5 isolates), ST220 (3 isolates), ST13, ST50, ST193, ST196, ST218 and ST221 (one isolate each).
Staphylococcus delphini and S. pseudintermedius are common nasal colonisers of donkeys, generally susceptible to the antimicrobials tested; nevertheless, these SIG isolates contain virulence genes, including the recently described exfoliative gene (expA) and several enterotoxin genes, with potential implications for public health. This is the first description of S. delphini in Tunisia. The Summary is available in Chinese - see Supporting information.
中间葡萄球菌属（SIG）细菌可在某些动物的鼻腔中定殖，但也是人和动物中新兴的病原体。
分析突尼斯用于食用的健康驴的SIG鼻腔携带情况，并对分离菌株进行特征分析。
对100头健康驴的鼻拭子进行SIG检测，并通过生化和分子方法鉴定分离菌株。检测分离菌株的抗菌药敏性，并检测抗菌耐药性和毒力基因。通过多位点序列分型和SmaI脉冲场凝胶电泳在克隆水平上对分离菌株进行分型。
分别在19%和2%的检测样本中获得了海豚葡萄球菌和伪中间葡萄球菌（属于SIG），每个样本鉴定出一株分离菌株。所有分离菌株均对甲氧西林敏感且mecA阴性。大多数海豚葡萄球菌和伪中间葡萄球菌分离菌株对所有检测的抗菌药物敏感，但有2株分离菌株对四环素（tet(M)基因）或夫西地酸耐药。鉴定出以下毒素基因（分离菌株的百分比）：lukS-I（100%）、lukF-I（9.5%）、siet（100%）、se-int（90%）、seccanine（19%）和expA（9.5%）。在21株SIG分离菌株中鉴定出13种不同的脉冲场凝胶电泳图谱。此外，通过多位点序列分型检测到以下9种不同的序列类型（STs），其中6种是新的：ST219（6株）、ST12（5株）、ST220（3株）、ST13、ST50、ST193、ST196、ST218和ST221（各1株）。
海豚葡萄球菌和伪中间葡萄球菌是驴常见的鼻腔定殖菌，通常对检测的抗菌药物敏感；然而，这些SIG分离菌株含有毒力基因，包括最近描述的剥脱毒素基因（expA）和几种肠毒素基因，对公共卫生有潜在影响。这是突尼斯首次对海豚葡萄球菌的描述。中文摘要见支持信息。
