Jin Yi, Tong Dayue, Shen Junjie, Yang Jianyong, Li Jiaping
Department of Pathology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
Tumour Biol. 2014 Sep;35(9):9079-83. doi: 10.1007/s13277-014-2161-0. Epub 2014 Jun 7.
Our aims were to investigate and establish simple and reliable implanted hepatocellular carcinoma (HCC) models in Wistar rats. Concentrated suspensions of CBRH-7919 cancer cell lines were injected subcutaneously into the scapular regions of nude mice. The developing tumor tissues were then implanted into the livers of 45 adult Wistar rats. Dexamethasone (2.5 mg/day) was injected intramuscularly daily for 1 week preoperatively and 2 weeks postoperatively. After 4 weeks of implantation, ultrasonography and nuclear magnetic resonance imaging (MRI) were performed to identify model rats with liver tumor growth and to analyze the growth and characteristics of the tumors. Five of these model rats were then sacrificed, and the tumors were removed from the liver for pathological examination. Three rats died during the operation; among the remaining 42 rats, 36 possessed a total of 43 liver tumors. The success rate of tumor implantation was 85.7 % (36/42), and the diameters of the tumors ranged from 5 to 10 mm. All tumor specimens were confirmed to be HCC by pathological examination. This study provides a new approach for establishing implanted HCC models in Wistar rats, which can be used for studying numerous biological features of HCC.
我们的目的是研究并建立Wistar大鼠简单可靠的植入性肝细胞癌（HCC）模型。将CBRH-7919癌细胞系的浓缩悬液皮下注射到裸鼠肩胛区域。然后将生长的肿瘤组织植入45只成年Wistar大鼠的肝脏。术前1周和术后2周每天肌肉注射地塞米松（2.5mg/天），持续1周。植入4周后，进行超声检查和核磁共振成像（MRI），以识别有肝肿瘤生长的模型大鼠，并分析肿瘤的生长情况和特征。然后处死其中5只模型大鼠，将肿瘤从肝脏中取出进行病理检查。手术中有3只大鼠死亡；在其余42只大鼠中，36只共有43个肝肿瘤。肿瘤植入成功率为85.7%（36/42），肿瘤直径为5至10mm。所有肿瘤标本经病理检查均确诊为HCC。本研究为在Wistar大鼠中建立植入性HCC模型提供了一种新方法，可用于研究HCC的多种生物学特性。
