Department of Spine Osteopathia, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, China.
College of Public Hygiene of Guangxi Medical University, Nanning, Guangxi 530021, China.
Mediators Inflamm. 2014;2014:786947. doi: 10.1155/2014/786947. Epub 2014 Apr 30.
In this study, we investigated the role of IL-17 via activation of STAT3 in the pathophysiology of SCI.
The purpose of the experiments is to study the expression of IL-17 and related cytokines via STAT3 signaling pathways, which is caused by the acute inflammatory response following SCI in different periods via establishing an acute SCI model in rat.
Basso, Beattie, and Bresnahan hind limb locomotor rating scale was used to assess the rat hind limb motor function. Immunohistochemistry was used to determine the expression levels of IL-17 and p-STAT3 in spinal cord tissues. Western blotting analysis was used to determine the protein expression of p-STAT3 in spinal cord tissue. RT-PCR was used to analyze the mRNA expression of IL-17 and IL-23p19 in the spleen tissue. ELISA was used to determine the peripheral blood serum levels of IL-6, IL-21, and IL-23.
Compared to the sham-operated group, the expression levels of IL-17, p-STAT3, IL-6, IL-21, and IL-23 were significantly increased and peaked at 24 h after SCI. The increased levels of cytokines were correlated with the SCI disease stages.
IL-17 may play an important role in promoting spinal cord neuroinflammation after SCI via activation of STAT3.
在这项研究中，我们通过激活 STAT3 研究了 IL-17 在 SCI 病理生理学中的作用。
实验的目的是通过建立大鼠急性 SCI 模型，研究不同时期 SCI 后急性炎症反应引起的 IL-17 及相关细胞因子通过 STAT3 信号通路的表达。
采用 Basso、Beattie 和 Bresnahan 后肢运动评分量表评估大鼠后肢运动功能。免疫组织化学法测定脊髓组织中 IL-17 和 p-STAT3 的表达水平。Western blot 分析测定脊髓组织中 p-STAT3 的蛋白表达。RT-PCR 分析脾组织中 IL-17 和 IL-23p19 的 mRNA 表达。ELISA 法测定外周血血清中 IL-6、IL-21 和 IL-23 的水平。
与假手术组相比，SCI 后 24 h，IL-17、p-STAT3、IL-6、IL-21 和 IL-23 的表达水平显著升高，并达到峰值。细胞因子的升高水平与 SCI 疾病阶段相关。
IL-17 通过激活 STAT3 可能在 SCI 后促进脊髓神经炎症中发挥重要作用。
