a Division of Livestock Products Technology , Indian Veterinary Research Institute , Izatnagar, Bareilly , Uttar Pradesh , India.
Crit Rev Food Sci Nutr. 2015;55(10):1303-14. doi: 10.1080/10408398.2012.690095.
Sex determination of domestic animal's meat is of potential value in meat authentication and quality control studies. Methods aiming at determining the sex origin of meat may be based either on the analysis of hormone or on the analysis of nucleic acids. At the present time, sex determination of meat and meat products based on hormone analysis employ gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS), and enzyme-linked immunosorbent assay (ELISA). Most of the hormone-based methods proved to be highly specific and sensitive but were not performed on a regular basis for meat sexing due to the technical limitations or the expensive equipments required. On the other hand, the most common methodology to determine the sex of meat is unquestionably traditional polymerase chain reaction (PCR) that involves gel electrophoresis of DNA amplicons. This review is intended to provide an overview of the DNA-based methods for sex determination of meat and meat products.
家畜肉的性别鉴定在肉类鉴定和质量控制研究中具有潜在价值。用于确定肉的性别来源的方法可以基于激素分析或核酸分析。目前，基于激素分析的肉类和肉类产品的性别鉴定采用气相色谱-质谱联用（GC-MS）、高效液相色谱-质谱/质谱联用（HPLC-MS/MS）和酶联免疫吸附测定（ELISA）。由于技术限制或所需昂贵设备的原因，大多数基于激素的方法被证明具有高度特异性和敏感性，但并未常规用于肉类性别鉴定。另一方面，确定肉类性别的最常见方法无疑是传统的聚合酶链反应（PCR），涉及 DNA 扩增子的凝胶电泳。本文旨在概述肉类和肉类产品性别鉴定的基于 DNA 的方法。
