Meimetis Labros G, Carlson Jonathan C T, Giedt Randy J, Kohler Rainer H, Weissleder Ralph
Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114 (USA).
Angew Chem Int Ed Engl. 2014 Jul 14;53(29):7531-4. doi: 10.1002/anie.201403890. Epub 2014 Jun 10.
We have developed a series of new ultrafluorogenic probes in the blue-green region of the visible-light spectrum that display fluorescence enhancement exceeding 11,000-fold. These fluorogenic dyes integrate a coumarin fluorochrome with the bioorthogonal trans-cyclooctene(TCO)-tetrazine chemistry platform. By exploiting highly efficient through-bond energy transfer (TBET), these probes exhibit the highest brightness enhancements reported for any bioorthogonal fluorogenic dyes. No-wash, fluorogenic imaging of diverse targets including cell-surface receptors in cancer cells, mitochondria, and the actin cytoskeleton is possible within seconds, with minimal background signal and no appreciable nonspecific binding, opening the possibility for in vivo sensing.
我们开发了一系列新的超荧光探针，它们在可见光光谱的蓝绿色区域显示出超过11000倍的荧光增强。这些荧光染料将香豆素荧光团与生物正交反式环辛烯（TCO）-四嗪化学平台相结合。通过利用高效的键间能量转移（TBET），这些探针展现出了任何生物正交荧光染料所报道的最高亮度增强。无需洗涤，数秒内即可对包括癌细胞中的细胞表面受体、线粒体和肌动蛋白细胞骨架在内的多种靶点进行荧光成像，背景信号极低且无明显的非特异性结合，为体内传感开辟了可能性。
