Takahashi Ryoichi, Shimizu Kazuo, Numabe Yukihiro
Department of Periodontology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan,
Odontology. 2015 May;103(2):194-202. doi: 10.1007/s10266-014-0157-2. Epub 2014 Jun 12.
The purpose of this research was to clarify the effects of microplasma irradiation on human gingival fibroblasts (HGF). Microplasma irradiation exposure for all HGF samples was limited to 30 s at an irradiation distance of 10 mm with a gas flow of 10 L/min. Three experimental groups were used: a 0 V control group (Control); a 650 V (low) microplasma irradiation group (LV); and a 975 V (high) irradiation group (HV). The following cellular characteristics were evaluated in order to analyze the effects of microplasma treatment; morphology, cell count, DNA content, metabolic activity, cell migration, fibroblast growth factor β (FGF-2) production, type I collagen secretion, and cytotoxic analysis. Cell count, DNA content and FGF-2 production have all been linked to wound healing and, interestingly, both the LV and HV groups showed significant (P < 0.05) increases in these categories at 72 h after irradiation when compared to the control group. Cytotoxic effects were measured by determining the levels of lactate dehydrogenase, cell death, and DNA damage in HGF cells. In these analyses, the HV and LV groups were not statistically different when compared with the control group at 72 h post-irradiation. These findings suggest that microplasma irradiation activated HGF with no clear cell-damaging effects.
本研究的目的是阐明微等离子体辐照对人牙龈成纤维细胞（HGF）的影响。所有HGF样本的微等离子体辐照暴露在辐照距离为10 mm、气流为10 L/min的条件下限制为30 s。使用了三个实验组：0 V对照组（Control）；650 V（低）微等离子体辐照组（LV）；以及975 V（高）辐照组（HV）。为了分析微等离子体处理的效果，评估了以下细胞特征：形态、细胞计数、DNA含量、代谢活性、细胞迁移、成纤维细胞生长因子β（FGF-2）产生、I型胶原分泌和细胞毒性分析。细胞计数、DNA含量和FGF-2产生都与伤口愈合有关，有趣的是，与对照组相比，LV组和HV组在辐照后72 h时这些指标均有显著（P < 0.05）增加。通过测定HGF细胞中乳酸脱氢酶水平、细胞死亡和DNA损伤来测量细胞毒性作用。在这些分析中，辐照后72 h时，HV组和LV组与对照组相比无统计学差异。这些发现表明微等离子体辐照激活了HGF，且没有明显的细胞损伤作用。
