Franceschini Emilie, Guillermin Régine, Tourniaire Franck, Roffino Sandrine, Lamy Edouard, Landrier Jean-François
Laboratoire de Mécanique et d'Acoustique LMA - CNRS UPR 7051, Aix-Marseille Université, Centrale Marseille, 13402 Marseille Cedex 20, France.
Laboratoire Motricité Humaine Education Sport Santé LAMHESS, Université de Nice Sophia-Antipolis, Nice, France.
J Acoust Soc Am. 2014 Jun;135(6):3620-31. doi: 10.1121/1.4876375.
Ultrasonic backscatter coefficient (BSC) measurements were performed on K562 cell pellet biophantoms with cell concentrations ranging from 0.006 to 0.30 in the 10-42 MHz frequency bandwidth. Three scattering models, namely, the fluid-filled sphere model (FFSM), the particle model (PM), and the structure factor model (SFM), were compared for modeling the scattering from an ensemble of concentrated cells. A parameter estimation procedure was developed in order to estimate the scatterer size and relative impedance contrast that could explain the measured BSCs from all the studied cell concentrations. This procedure was applied to the BSC data from K562 cell pellet biophantoms in the 10-42 MHz frequency bandwidth and to the BSC data from Chinese hamster ovary cell pellet biophantoms in the 26-105 MHz frequency bandwidth given in Han, Abuhabsah, Blue, Sarwate, and O'Brien [J. Acoust. Soc. Am. 130, 4139-4147 (2011)]. The data fitting quality and the scatterer size estimates show that the SFM was more suitable than the PM and the FFSM for modeling the responses from concentrated cell pellet biophantoms.
在频率带宽为10 - 42MHz的条件下，对细胞浓度范围为0.006至0.30的K562细胞沉淀生物仿体进行了超声背向散射系数（BSC）测量。比较了三种散射模型，即充液球体模型（FFSM）、粒子模型（PM）和结构因子模型（SFM），以模拟来自浓缩细胞集合的散射。开发了一种参数估计程序，以估计散射体大小和相对阻抗对比度，从而能够解释所有研究细胞浓度下测得的BSC。该程序应用于频率带宽为10 - 42MHz的K562细胞沉淀生物仿体的BSC数据，以及Han、Abuhabsah、Blue、Sarwate和O'Brien [《美国声学学会杂志》130, 4139 - 4147 (2011)] 中给出的频率带宽为26 - 105MHz的中国仓鼠卵巢细胞沉淀生物仿体的BSC数据。数据拟合质量和散射体大小估计表明，SFM比PM和FFSM更适合模拟浓缩细胞沉淀生物仿体的响应。
