纳米点阵列对 C6 神经胶质瘤细胞-细胞相互作用的地形控制。
Topographical control of cell-cell interaction in C6 glioma by nanodot arrays.
机构信息
Department of Materials Science and Engineering, National Chiao Tung University, 1001 University Road, Hsinchu 300, Taiwan.
Department of Materials Science and Engineering, National Chiao Tung University, 1001 University Road, Hsinchu 300, Taiwan ; Institute of Biomedical Engineering, National Chiao Tung University, 1001 University Road, Hsinchu 300, Taiwan.
出版信息
Nanoscale Res Lett. 2014 May 21;9(1):250. doi: 10.1186/1556-276X-9-250. eCollection 2014.
Nanotopography modulates the physiological behavior of cells and cell-cell interactions, but the manner of communication remains unclear. Cell networking (syncytium) of astroglia provides the optimal microenvironment for communication of the nervous system. C6 glioma cells were seeded on nanodot arrays with dot diameters ranging from 10 to 200 nm. Cell viability, morphology, cytoskeleton, and adhesion showed optimal cell growth on 50-nm nanodots if sufficient incubation was allowed. In particular, the astrocytic syncytium level maximized at 50 nm. The gap junction protein Cx43 showed size-dependent and time-dependent transport from the nucleus to the cell membrane. The transport efficiency was greatly enhanced by incubation on 50-nm nanodots. In summary, nanotopography is capable of modulating cell behavior and influencing the cell-cell interactions of astrocytes. By fine-tuning the nanoenvironment, it may be possible to regulate cell-cell communications and optimize the biocompatibility of neural implants.
纳米形貌调节细胞的生理行为和细胞间相互作用，但通讯方式仍不清楚。星形胶质细胞的细胞网络（合胞体）为神经系统的通讯提供了最佳的微环境。将 C6 神经胶质瘤细胞接种在直径为 10 至 200nm 的纳米点阵列上。如果允许足够的孵育，细胞活力、形态、细胞骨架和黏附在 50nm 纳米点上表现出最佳的细胞生长。特别是，星形胶质细胞合胞体水平在 50nm 时达到最大值。缝隙连接蛋白 Cx43 表现出依赖于尺寸和时间的从核到质膜的转运。在 50nm 纳米点上孵育可大大提高转运效率。总之，纳米形貌能够调节细胞行为并影响星形胶质细胞的细胞间相互作用。通过微调纳米环境，可能可以调节细胞间通讯并优化神经植入物的生物相容性。
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