一种与DNA稳定相连的进化保守蛋白质组分。

An evolutionarily conserved protein fraction stably linked to DNA.

作者信息

Avramova Z, Mikhailov I, Tsanev R

机构信息

Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.

出版信息

Biochim Biophys Acta. 1989 Jan 23;1007(1):109-11. doi: 10.1016/0167-4781(89)90137-1.

DOI:10.1016/0167-4781(89)90137-1

PMID:2491788

Abstract

Chromatins from four evolutionarily remote species (insect, fish, amphibian and bird) were isolated, high-salt-extracted and extensively deproteinized to remove noncovalently associated proteins. A protein fraction resisting the extraction procedures was found firmly linked to DNA in all four chromatins. Two-dimensional tryptic peptide mapping revealed a remarkable evolutionary conservativeness of this protein component, suggesting an indispensable function for it in the nucleus.

摘要

从四个进化上相距甚远的物种（昆虫、鱼类、两栖动物和鸟类）中分离出染色质，进行高盐提取并大量去除蛋白质以除去非共价结合的蛋白质。在所有四种染色质中，均发现一种抵抗提取过程的蛋白质组分与DNA紧密相连。二维胰蛋白酶肽图谱显示该蛋白质组分具有显著的进化保守性，表明其在细胞核中具有不可或缺的功能。