Institute of Agrophysics, Polish Academy of Sciences, Doswiadczalna 4, 20-290 Lublin, Poland.
Plant Methods. 2014 May 29;10:14. doi: 10.1186/1746-4811-10-14. eCollection 2014.
The primary cell wall of fruits and vegetables is a structure mainly composed of polysaccharides (pectins, hemicelluloses, cellulose). Polysaccharides are assembled into a network and linked together. It is thought that the percentage of components and of plant cell wall has an important influence on mechanical properties of fruits and vegetables.
In this study the Raman microspectroscopy technique was introduced to the visualization of the distribution of polysaccharides in cell wall of fruit. The methodology of the sample preparation, the measurement using Raman microscope and multivariate image analysis are discussed. Single band imaging (for preliminary analysis) and multivariate image analysis methods (principal component analysis and multivariate curve resolution) were used for the identification and localization of the components in the primary cell wall.
Raman microspectroscopy supported by multivariate image analysis methods is useful in distinguishing cellulose and pectins in the cell wall in tomatoes. It presents how the localization of biopolymers was possible with minimally prepared samples.
水果和蔬菜的初生细胞壁主要由多糖（果胶、半纤维素、纤维素）组成的结构。多糖组装成网络并连接在一起。人们认为成分的百分比和植物细胞壁的百分比对水果和蔬菜的机械性能有重要影响。
本研究将拉曼微光谱技术引入到水果细胞壁中多糖分布的可视化研究中。讨论了样品制备的方法学、拉曼显微镜的测量和多元图像分析。使用单波段成像（初步分析）和多元图像分析方法（主成分分析和多元曲线分辨）来鉴定和定位初生细胞壁中的成分。
多元图像分析支持的拉曼微光谱技术可用于区分番茄细胞壁中的纤维素和果胶。它展示了如何在最小预处理样品的情况下进行生物聚合物的定位。
