The London School of Hygiene & Tropical Medicine, Keppel St, London WC1E 7HT, United Kingdom ; School of Life Sciences, Faculty of Science, Engineering and Computing, Kingston University, Kingston upon Thames, Penrhyn Road, KT1 2EE, United Kingdom.
The London School of Hygiene & Tropical Medicine, Keppel St, London WC1E 7HT, United Kingdom.
FEBS Open Bio. 2014 May 9;4:468-72. doi: 10.1016/j.fob.2014.04.012. eCollection 2014.
According to MEROPS peptidase database, Campylobacter species encode 64 predicted peptidases. However, proteolytic properties of only a few of these proteins have been confirmed experimentally. In this study we identified and characterised a Campylobacter jejuni gene cj0511 encoding a novel peptidase. The proteolytic activity associated with this enzyme was demonstrated in cell lysates. Moreover, enzymatic studies conducted with a purified protein confirmed a prediction of it being a serine peptidase. Furthermore, cj0511 mutant was found to be severely attenuated in chicken colonisation model, suggesting a role of the Cj0511 protein in infection.
根据 MEROPS 肽酶数据库，弯曲杆菌属物种编码 64 种预测的肽酶。然而，这些蛋白质中只有少数几种的蛋白水解特性已被实验证实。在这项研究中，我们鉴定并表征了编码一种新型肽酶的空肠弯曲杆菌 cj0511 基因。该酶相关的蛋白水解活性在细胞裂解物中得到了证实。此外，对纯化蛋白进行的酶学研究证实了它是一种丝氨酸肽酶的预测。此外，在鸡定植模型中发现 cj0511 突变体严重减毒，表明 Cj0511 蛋白在感染中起作用。
