Umemoto Hiroki, Sawada Keigo, Kurata Atsushi, Hamaguchi Shohei, Tsukahara Satoshi, Ishiguro Takashi, Kishimoto Noriaki
Department of Applied Biological Chemistry, Kinki University.
J Oleo Sci. 2014;63(7):671-9. doi: 10.5650/jos.ess14029. Epub 2014 Jun 11.
A high-nervonic acid (cis-15-tetracosenoic acid, C24:1, n-9)-producing filamentous fungus of the Mortierella species was discovered among soil filamentous fungi. The filamentous fungal strain -RD000969- was isolated from soil collected in Kanagawa Prefecture (Japan) and was found to accumulate nervonic acid at a rate of 6.94% of the total cellular fatty acids. The base sequences of 28S rDNA D1/D2 and ITS 5.8S rDNA showed 100% homology with Mortierella capitata CBS 293.96. In addition to nervonic acid, strain RD000969 produced a large amount of long-chain monounsaturated fatty acids (C20:1, 12.22%; C22:1, 4.07%; C26:1, 5.91%) and a small amount of ultra-long-chain fatty acids (C28:1, 0.44%; C30:1, 0.06%; C32:1, trace). In the fungal cells, 98.87% of nervonic acid was localized at the sn-1,3 position of triacylglycerol. Nervonic acid production was maximum (186.3 mg·L(-1)) when the fungus was cultured in potato dextrose (PD) medium containing yeast extract, CaCl2, and MgSO4·7H2O.
在土壤丝状真菌中发现了一种能高产神经酸（顺式-15-二十四碳烯酸，C24:1，n-9）的被孢霉属丝状真菌。该丝状真菌菌株-RD000969-从日本神奈川县采集的土壤中分离得到，发现其神经酸积累量占细胞总脂肪酸的6.94%。28S rDNA D1/D2和ITS 5.8S rDNA的碱基序列与头状被孢霉CBS 293.96显示出100%的同源性。除神经酸外，菌株RD000969还产生大量长链单不饱和脂肪酸（C20:1，12.22%；C22:1，4.07%；C26:1，5.91%）和少量超长链脂肪酸（C28:1，0.44%；C30:1，0.06%；C32:1，痕量）。在真菌细胞中，98.87%的神经酸位于三酰甘油的sn-1,3位。当该真菌在含有酵母提取物、CaCl2和MgSO4·7H2O的马铃薯葡萄糖（PD）培养基中培养时，神经酸产量最高（186.3 mg·L(-1)）。
