Tang Yunlian, Luo Chunyan, Cheng Ailan, Lu Suli, Xu Jinhua, Fu Ting, Gan Runliang
Cancer Research Institute, University of South China, Hengyang, Hunan 421001, P.R. China.
Mol Med Rep. 2014 Aug;10(2):1117-21. doi: 10.3892/mmr.2014.2313. Epub 2014 Jun 10.
Infection with Epstein-Barr virus (EBV) induces activation and proliferation of B lymphocytes. Detection of latent membrane protein (LMP)-1 is used to identify the proliferative ability of B cells. However, changes in the expression levels of the three LMPs during EBV-induced B lymphocyte transformation, have not yet been reported. In the present study, the expression levels of LMP-1, LMP-2A and LMP-2B were compared between EBV-transformed B lymphocytes and paired normal lymphocytes. Seven lymphoblast cell lines were established by EBV infection of normal human lymphocytes in vitro. The expression levels of LMP genes and LMP-1 protein were determined using quantitative (q)PCR and western blotting in lymphoblasts and normal lymphocytes, respectively. The expression of LMP1, LMP-2A and LMP-2B genes was significantly upregulated in EBV-induced lymphoblasts compared with the normal lymphocytes. The LMP-1 protein level was also significantly increased in EBV-transformed B lymphocytes. Expression of LMP1, LMP-2A and LMP-2B genes was significantly upregulated in EBV-induced lymphoblasts, suggesting LMP genes are important in the transformation of human lymphocytes.
感染爱泼斯坦-巴尔病毒（EBV）会诱导B淋巴细胞的激活和增殖。检测潜伏膜蛋白（LMP）-1可用于鉴定B细胞的增殖能力。然而，EBV诱导B淋巴细胞转化过程中三种LMP表达水平的变化尚未见报道。在本研究中，比较了EBV转化的B淋巴细胞与配对的正常淋巴细胞中LMP-1、LMP-2A和LMP-2B的表达水平。通过体外EBV感染正常人淋巴细胞建立了7个淋巴母细胞系。分别使用定量（q）PCR和蛋白质印迹法测定淋巴母细胞和正常淋巴细胞中LMP基因和LMP-1蛋白的表达水平。与正常淋巴细胞相比，EBV诱导的淋巴母细胞中LMP1、LMP-2A和LMP-2B基因的表达显著上调。EBV转化的B淋巴细胞中LMP-1蛋白水平也显著升高。EBV诱导的淋巴母细胞中LMP1、LMP-2A和LMP-2B基因的表达显著上调，提示LMP基因在人类淋巴细胞转化中起重要作用。
