Chen Kai, Li Zhonghu, Jiang Peng, Zhang Xi, Zhang Yujun, Jiang Yan, He Yu, Li Xiaowu
Department of Hepatobiliary Surgery Institute, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R. China.
Oncol Rep. 2014 Aug;32(2):755-63. doi: 10.3892/or.2014.3245. Epub 2014 Jun 11.
The metastasis-related molecules CD133, CD44v6 and human tissue factor (TF) have been shown to be associated with tumor invasion and metastasis. This study aimed to determine whether co-expression of these three molecules was associated with metastasis and overall prognosis in pancreatic carcinoma. We analyzed the expression profiles of these three molecules by immunohistochemistry and evaluated the relationship of their expression profiles with metastasis and prognosis in 109 pancreatic carcinomas. The results showed that the expression levels of CD133, CD44v6 and TF were increased in pancreatic carcinoma. Co-expression of CD133, CD44v6 and TF (tri-expression) was also detected in pancreatic carcinoma. Clinical analysis showed that individual expression of CD133, CD44v6 or TF was associated with vessel invasion, lymph node metastasis and liver metastasis, while tri-expression was associated with lymph node metastasis. Survival analysis showed that patients with co-expression of CD133 and TF or tri-expression had lower and the lowest overall survival rates, respectively. Univariate analysis showed that T-factor, lymph node metastasis, TNM stage, and individual levels or tri-expression of CD133, CD44v6 and TF were survival risk factors. Multivariate analysis showed that tri-expression of CD133, CD44v6 and TF was an independent predictor of survival. These results suggest that overexpression of CD133, CD44v6 and TF is associated with pancreatic carcinoma metastasis. Tri-expression of these three molecules may be a useful predictor for pancreatic carcinoma prognosis.
转移相关分子CD133、CD44v6和人组织因子（TF）已被证明与肿瘤侵袭和转移有关。本研究旨在确定这三种分子的共表达是否与胰腺癌的转移及总预后相关。我们通过免疫组织化学分析了这三种分子的表达谱，并评估了它们的表达谱与109例胰腺癌转移及预后的关系。结果显示，胰腺癌中CD133、CD44v6和TF的表达水平升高。胰腺癌中还检测到CD133、CD44v6和TF的共表达（三表达）。临床分析表明，CD133、CD44v6或TF的单独表达与血管侵犯、淋巴结转移和肝转移相关，而三表达与淋巴结转移相关。生存分析表明，CD133和TF共表达或三表达的患者总体生存率分别较低和最低。单因素分析表明，T分期、淋巴结转移、TNM分期以及CD133、CD44v6和TF的单独水平或三表达是生存危险因素。多因素分析表明，CD133、CD44v6和TF的三表达是生存的独立预测因子。这些结果提示，CD133、CD44v6和TF的过表达与胰腺癌转移相关。这三种分子的三表达可能是胰腺癌预后的有用预测指标。
