CD44v/CD44s 的表达模式与胰腺癌患者的生存情况相关。
CD44v/CD44s expression patterns are associated with the survival of pancreatic carcinoma patients.
机构信息
Department of Hepatobiliary Surgery Institute, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China.
出版信息
Diagn Pathol. 2014 Apr 8;9:79. doi: 10.1186/1746-1596-9-79.
BACKGROUND AND PURPOSE
CD44 variants have been associated with tumor invasion and metastasis, but CD44 expression patterns have not been systematically investigated in pancreatic carcinoma. This study systematically investigated whether CD44 expression patterns are involved in pancreatic carcinoma metastasis and prognosis.
METHODS
We applied primers specific for all CD44 variants and CD44s to analyze the expression patterns of CD44 (CD44v2-CD44v10 and CD44s) using quantitative real-time PCR (qRT-PCR). We then further evaluated their roles in pancreatic carcinoma metastasis and prognosis using clinical survival analysis.
RESULTS
Increased CD44v expression and decreased CD44s expression were found in metastatic pancreatic carcinoma in three different cell lines and in human tumor tissue. Clinical analysis showed that CD44v6+ and CD44v9+ were correlated with lymph node metastasis, liver metastasis and TNM stage. However, CD44s- was associated with liver metastasis, tumor differentiation and TNM stage. Survival analysis showed that patients with CD44v6+/CD44s- or CD44v6+/CD44s- had lower overall survival (OS) rates, although the individual expression of CD44v6, CD44v9 and CD44s was also related to decreased OS rates. Univariate analysis showed that lymph node metastasis; vessel invasion; hepatic metastases; TNM stage; and individual or co-expression of CD44v6, CD44v9 and CD44s were risk factors affecting survival. Multivariate analysis showed that CD44v6+/CD44s- was an independent predictor of survival.
CONCLUSIONS
We found that CD44v6+, CD44v9+ and CD44s- were associated with pancreatic carcinoma metastasis and progression and that CD44v6+/CD44s- was an independent risk factor affecting survival in pancreatic carcinoma. Therefore, the different expression patterns of CD44v/CD44s may determine pancreatic carcinoma prognosis.
VIRTUAL SLIDES
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1579257224116287.
背景与目的
CD44 变体与肿瘤侵袭和转移有关，但 CD44 的表达模式尚未在胰腺癌中得到系统研究。本研究系统研究了 CD44 表达模式是否参与胰腺癌转移和预后。
方法
我们应用针对所有 CD44 变体和 CD44s 的引物，通过定量实时 PCR(qRT-PCR)分析 CD44(CD44v2-CD44v10 和 CD44s)的表达模式。然后，我们使用临床生存分析进一步评估它们在胰腺癌转移和预后中的作用。
结果
在三种不同的细胞系和人肿瘤组织中，转移性胰腺癌中发现 CD44v 表达增加，CD44s 表达减少。临床分析表明，CD44v6+和 CD44v9+与淋巴结转移、肝转移和 TNM 分期相关。然而，CD44s-与肝转移、肿瘤分化和 TNM 分期相关。生存分析表明，CD44v6+/CD44s-或 CD44v6+/CD44s-的患者总生存率(OS)较低，尽管 CD44v6、CD44v9 和 CD44s 的单独表达也与 OS 降低相关。单因素分析表明，淋巴结转移；血管侵犯；肝转移；TNM 分期；以及 CD44v6、CD44v9 和 CD44s 的个体或共同表达是影响生存的危险因素。多因素分析表明，CD44v6+/CD44s-是生存的独立预测因子。
结论
我们发现 CD44v6+、CD44v9+和 CD44s-与胰腺癌转移和进展相关，CD44v6+/CD44s-是影响胰腺癌生存的独立危险因素。因此，CD44v/CD44s 的不同表达模式可能决定胰腺癌的预后。
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