Sivagnanavelmurugan Madasamy, Thaddaeus Bergmans Jude, Palavesam Arunachalam, Immanuel Grasian
Marine Biotechnology Division, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam 629 502, Kanyakumari District, Tamilnadu, India.
Marine Biotechnology Division, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam 629 502, Kanyakumari District, Tamilnadu, India.
Fish Shellfish Immunol. 2014 Aug;39(2):439-49. doi: 10.1016/j.fsi.2014.05.037. Epub 2014 Jun 9.
The polysaccharide fucoidan from brown seaweed Sargassum wightii was extracted and it was incorporated with pellet diets at three concentrations (0.1, 0.2 & 0.3%). The fucoidan incorporated diets were fed to shrimp Penaeus monodon for 60 days and the growth performance was assessed. The weight gain and SGR of control group was 6.83 g and 9.72%, respectively, but the weight gain and SGR of various concentrations (0.1-0.3%) of fucoidan incorporated diets fed groups of shrimp was increased from 7.30 to 8.20 g and 9.83 to 10.03%, respectively. After 60 days of feeding experiment, the relative quantification of prophenoloxidase gene of experimental groups over control group was analysed by RT-PCR and it was ranged between 2.13 and 7.95 fold increase within 33.52-34.61 threshold cycles, respectively at 0.1-0.3% concentrations of fucoidan. After 60 days of feeding experiment, the P. monodon were challenged with shrimp pathogen Vibrio parahaemolyticus and the mortality percentage was recorded daily up to 21 days. The reduction in mortality percentage of experimental groups over control group was recorded from 44.56 to 72.79%, respectively in 0.1-0.3% of fucoidan incorporated diets fed groups. During challenge experiment, all the immunological parameters such as THC, prophenoloxidase activity, respiratory burst activity, superoxide dismutase activity, phagocytic activity, bactericidal activity and bacterial clearance ability of experimental groups were significantly (P < 0.05) increased than control group. The V. parahaemolyticus load was enumerated from the infected shrimp at every 10 days intervals during challenge experiment. In control group, the Vibrio load was increased in hepatopancreas and muscle tissues from 10th to 21st days of challenge test. But in the experimental groups, the Vibrio load in both the tissues decreased positively from 10th to 21st days of challenge duration. It is concluded that the S. wightii fucoidan had enhanced the innate immunity and increased resistance to V. parahaemolyticus infection in P. monodon.
从褐藻半叶马尾藻中提取了多糖岩藻聚糖，并将其以三种浓度（0.1%、0.2%和0.3%）添加到颗粒饲料中。将添加了岩藻聚糖的饲料投喂斑节对虾60天，并评估其生长性能。对照组的体重增加量和特定生长率分别为6.83克和9.72%，但投喂不同浓度（0.1 - 0.3%）岩藻聚糖饲料的对虾组的体重增加量和特定生长率分别从7.30克增加到8.20克，从9.83%增加到10.03%。在60天的投喂实验后，通过RT-PCR分析实验组相对于对照组的前酚氧化酶基因的相对定量，在0.1 - 0.3%浓度的岩藻聚糖下，其在33.52 - 34.61个阈值循环内分别增加了2.13至7.95倍。在60天的投喂实验后，用对虾病原菌副溶血性弧菌感染斑节对虾，并记录直至21天的每日死亡率。在投喂0.1 - 0.3%岩藻聚糖饲料的实验组中，相对于对照组，死亡率降低了44.56%至72.79%。在攻毒实验期间，实验组的所有免疫参数，如总血细胞计数、前酚氧化酶活性、呼吸爆发活性、超氧化物歧化酶活性、吞噬活性、杀菌活性和细菌清除能力均显著（P < 0.05）高于对照组。在攻毒实验期间，每隔10天对感染的对虾中的副溶血性弧菌载量进行计数。在对照组中，攻毒试验第10天至21天，肝胰腺和肌肉组织中的弧菌载量增加。但在实验组中，在攻毒持续时间的第10天至21天，两个组织中的弧菌载量均呈正向下降。得出结论，半叶马尾藻岩藻聚糖增强了斑节对虾的先天免疫力，并提高了其对副溶血性弧菌感染的抵抗力。
