Galib Md Rejwanul Haque, Ghosh Alokesh Kumar, Sabbir Wasim
Fisheries and Marine Resource Technology Discipline, Khulna University, Khulna, Bangladesh.
Heliyon. 2024 Dec 31;11(1):e41583. doi: 10.1016/j.heliyon.2024.e41583. eCollection 2025 Jan 15.
The present study was conducted to evaluate the efficacy of extract against the white spot syndrome virus (WSSV) in black tiger shrimp () following oral administration . The methanol extract derived from the extraction was sprayed into feed at a concentration of 0.0 %, 0.05 % and 0.1 %. The feeding trial was continued for four weeks, following which the shrimps were injected with WSSV and mortality was checked two weeks after the trial. The findings revealed that shrimp fed with extract had significantly improved ( < 0.05) growth parameters (weight gain, specific growth rate) and feed utilization efficacy (less feed conversion ratio, high protein efficiency ratio) compared to the control group. The extract improved the immunity of shrimp significantly, as demonstrated by increased immunological parameters including total haemocyte count, prophenoloxidase activity, superoxide dismutase activity and up regulation of immune-related genes, which ultimately increased the disease resistance capability in against WSSV. Based on the experiment, 0.1 % extract had the highest growth and immune response against WSSV followed by Treatment 1 and control. Therefore, the extract of could be used as an immunostimulant with feed to boost the growth and resistance capacity of shrimp against WSSV as well as for the sustainable production of shrimp.
本研究旨在评估斑节对虾口服某提取物后对白斑综合征病毒（WSSV）的疗效。将提取得到的甲醇提取物以0.0%、0.05%和0.1%的浓度喷洒到饲料中。投喂试验持续四周，之后给对虾注射WSSV，并在试验两周后检查死亡率。结果显示，与对照组相比，投喂该提取物的对虾生长参数（体重增加、特定生长率）和饲料利用效率（较低的饲料转化率、较高的蛋白质效率比）有显著改善（P<0.05）。该提取物显著提高了对虾的免疫力，表现为免疫参数增加，包括总血细胞计数、酚氧化酶原活性、超氧化物歧化酶活性以及免疫相关基因的上调，这最终提高了斑节对虾对WSSV的抗病能力。基于实验，0.1%提取物对WSSV的生长和免疫反应最高，其次是处理1和对照组。因此，该提取物可用作饲料中的免疫刺激剂，以促进对虾的生长和对WSSV的抗性，以及实现对虾的可持续生产。
