Aras Mutan Hamdi, Sezer Ufuk, Erkilic Suna, Demir Tuncer, Dagli Seyda Nur
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Gaziantep University, Gaziantep, Turkiye.
Department of Periodontology, Faculty of Dentistry, Gaziantep University, Gaziantep, Turkiye.
Eur J Dent. 2013 Jul;7(3):310-314. doi: 10.4103/1305-7456.115415.
The aim of this study was to evaluate the effect of boron on 5-fluorouracil (5-FU)-induced oral mucositis in rats.
Sixty-four male Wistar albino rats were injected with 5-FU on days 1 and 3. The right cheek pouch mucosa was scratched with the tip of an 18-G needle, dragged twice in a linear movement, on days 3 and 5. The animals were randomly divided into two groups of 32: boron group (BG) and control group (CG). Rats in the CG did not receive any treatment, whereas the others were fed boron (3 mg·kg(-1)·day(-1)) by gavage. The animals were sacrificed on day 3 (n = 8), 6 (n = 8), 9 (n = 8), and 12 (n = 8), and the cheek pouch was removed for histopathological analysis.
On day 3, both groups showed necrosis and active inflammation, but the inflammation was mild in CG and moderate in BG. On day 6, both BG and CG showed necrosis; in the CG, there was moderate inflammation, and in the BG, there was severe inflammation and granulation tissue around the necrotic area. On day 9, re-epithelization began in both groups, and there were no differences between groups. Re-epithelization was complete in both groups on day 12.
We found no beneficial effect of boron in healing oral mucositis. Additional research is warranted to elucidate the pathogenic inflammatory mechanisms involved in mucositis and the prophylactic and therapeutic roles of antioxidants.
本研究旨在评估硼对5-氟尿嘧啶（5-FU）诱导的大鼠口腔黏膜炎的影响。
64只雄性Wistar白化大鼠在第1天和第3天注射5-FU。在第3天和第5天，用18G针头尖端刮擦右侧颊囊黏膜，呈直线移动刮擦两次。动物被随机分为两组，每组32只：硼组（BG）和对照组（CG）。CG组大鼠未接受任何治疗，而其他大鼠通过灌胃给予硼（3mg·kg⁻¹·天⁻¹）。在第3天（n = 8）、第6天（n = 8）、第9天（n = 8）和第12天（n = 8）处死动物，取出颊囊进行组织病理学分析。
第3天，两组均出现坏死和活动性炎症，但CG组炎症较轻，BG组炎症中度。第6天，BG组和CG组均出现坏死；CG组有中度炎症，BG组坏死区域周围有严重炎症和肉芽组织。第9天，两组均开始重新上皮化，组间无差异。第12天，两组重新上皮化均完成。
我们发现硼对口腔黏膜炎的愈合没有有益作用。有必要进行更多研究以阐明黏膜炎中涉及的致病炎症机制以及抗氧化剂的预防和治疗作用。
