Wu Tingting, Chen Chen, Li Fen, Chen Zhe, Xu Yong, Xiao Botui, Tao Zezhang
Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
Exp Ther Med. 2014 Jun;7(6):1635-1638. doi: 10.3892/etm.2014.1649. Epub 2014 Mar 28.
Nasopharyngeal carcinoma (NPC) is characterized by silent progression and atypical early symptoms. Early metastasis to the neck lymph nodes is common. However, conventional chemoradiotherapy is limited and unable to effectively control cervical lymph node metastasis of NPC. In addition, toxicities caused by chemoradiotherapy often induce damage to normal tissues and organs. Thus, the aim of this study was to investigate the ability of 3,3'-diindolylmethane (DIM) to inhibit the invasion and metastasis of NPC cells and . The migration and invasive abilities of the 5-8F human NPC cell line were detected using a Transwell assay. Lymph node metastasis in nude mice was observed following the implantation of xenograft tumors for 8 weeks. In addition, western blot analysis was used to detect the expression levels of epithelial mesenchymal transition (EMT)-associated key proteins in NPC cells treated with DIM and . The results demonstrated that DIM effectively inhibited the migration and invasion of NPC cells and the effect was concentration-dependent. In addition, DIM significantly delayed and reduced the occurrence of lymph node metastasis in the animal model. The expression levels of a number of key proteins associated with EMT were affected by DIM treatment. In the animal model, there were no signs of toxicity in the vital organs, including the heart, liver and kidney, of animals fed a diet containing DIM. Therefore, the results of the present study indicate that DIM affects the expression levels of a number of EMT-associated key proteins and induces the inhibition of invasion and metastasis of NPC cells and .
鼻咽癌（NPC）的特点是进展隐匿且早期症状不典型。早期转移至颈部淋巴结很常见。然而，传统的放化疗存在局限性，无法有效控制鼻咽癌的颈部淋巴结转移。此外，放化疗引起的毒性常常导致正常组织和器官受损。因此，本研究的目的是探讨3,3'-二吲哚甲烷（DIM）抑制NPC细胞侵袭和转移的能力。使用Transwell实验检测5-8F人NPC细胞系的迁移和侵袭能力。在裸鼠体内植入异种移植瘤8周后观察淋巴结转移情况。此外，采用蛋白质免疫印迹分析检测经DIM处理的NPC细胞中上皮-间质转化（EMT）相关关键蛋白的表达水平。结果表明，DIM有效抑制了NPC细胞的迁移和侵袭，且该作用呈浓度依赖性。此外，DIM显著延迟并减少了动物模型中淋巴结转移的发生。多种与EMT相关的关键蛋白的表达水平受DIM处理的影响。在动物模型中，喂食含DIM饮食的动物的心、肝、肾等重要器官未出现毒性迹象。因此，本研究结果表明，DIM影响多种与EMT相关的关键蛋白的表达水平，并诱导抑制NPC细胞的侵袭和转移。
