肾移植受者中不同类型CD4(+)Foxp3(+) T细胞的失衡

Imbalance of different types of CD4(+)Foxp3(+) T cells in renal transplant recipients.

作者信息

Hu Kebang, Zhou Honglan, Zheng Guoyou, Wang Gang, Fu Yaowen, Jiang Yanfang

机构信息

Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital of Jilin University , Changchun , China and.

出版信息

Immunol Invest. 2014;43(8):838-50. doi: 10.3109/08820139.2014.909458. Epub 2014 Jun 13.

DOI:10.3109/08820139.2014.909458

PMID:24927378

Abstract

AIMS

To determine the number of CD4(+)CD25(-)Foxp3(+), CD4(+)CD25(+)Foxp3(+) and CD4(+)CXCR5(+)Foxp3(+) T cells in renal transplant recipients that are transplanted stable (TS), or experiencing accelerated rejection (ALR), or acute rejection (AR).

METHODS

Renal transplantation was conducted in 28 patients with end-stage renal failure (ESRF). The number of peripheral CD4(+)CD25(-)Foxp3(+), CD4(+)CD25(+)Foxp3(+), or CD4(+)CXCR5(+)Foxp3(+) T cells and the serum levels of interleukin-10 (IL-10) were measured in pre- and post-transplant patients and these results were compared to 10 healthy controls (HC). Correlation between CD4(+)CD25(+)Foxp3(+) and estimated glomerular filtration rate (eGFR), CD4(+)CD25(-)Foxp3(+) and serum creatinine (Cr) levels, or Cr and IL-10 levels in TS patients was also determined.

RESULTS

The number of CD4(+)CD25(-)Foxp3(+) T cells was significantly increased in patients with ESRF, as compared to HC. Stratification analysis demonstrated that TS patients contained greater numbers of CD4(+)CD25(+)Foxp3(+) and CD4(+)CXCR5(+)Foxp3(+) T cells, higher levels of serum IL-10, and fewer numbers of CD4(+)CD25(-)Foxp3(+) T cells than ESRF patients. In contrast, ALR and AR patients contained fewer numbers of CD4(+)CD25(+)Foxp3(+) and CD4(+)CXCR5(+)Foxp3(+) T cells, greater numbers of CD4(+)CD25(-)Foxp3(+) T cells, and lower levels of serum IL-10 than ESRF patients. In TS patients, the numbers of CD4(+)CD25(+)Foxp3(+) and CD4(+)CD25(-)Foxp3(+) T cells were positively correlated with eGFR and serum Cr levels, respectively.

CONCLUSION

An imbalance of different types of CD4(+)Foxp3(+) T cells might be involved in renal transplant rejection.

摘要

目的

确定肾移植稳定（TS）、加速排斥（ALR）或急性排斥（AR）的肾移植受者中CD4(+)CD25(-)Foxp3(+)、CD4(+)CD25(+)Foxp3(+)和CD4(+)CXCR5(+)Foxp3(+) T细胞的数量。

方法

对28例终末期肾衰竭（ESRF）患者进行肾移植。检测移植前后患者外周血中CD4(+)CD25(-)Foxp3(+)、CD4(+)CD25(+)Foxp3(+)或CD4(+)CXCR5(+)Foxp3(+) T细胞数量以及血清白细胞介素-10（IL-10）水平，并将这些结果与10名健康对照者（HC）进行比较。还确定了TS患者中CD4(+)CD25(+)Foxp3(+)与估计肾小球滤过率（eGFR）、CD4(+)CD25(-)Foxp3(+)与血清肌酐（Cr）水平或Cr与IL-10水平之间的相关性。

结果

与HC相比，ESRF患者中CD4(+)CD25(-)Foxp3(+) T细胞数量显著增加。分层分析表明，与ESRF患者相比，TS患者含有更多数量的CD4(+)CD25(+)Foxp3(+)和CD4(+)CXCR5(+)Foxp3(+) T细胞、更高水平的血清IL-10以及更少数量的CD4(+)CD25(-)Foxp3(+) T细胞。相反，与ESRF患者相比，ALR和AR患者含有更少数量的CD4(+)CD25(+)Foxp3(+)和CD4(+)CXCR5(+)Foxp3(+) T细胞、更多数量的CD4(+)CD25(-)Foxp3(+) T细胞以及更低水平的血清IL-10。在TS患者中，CD4(+)CD25(+)Foxp3(+)和CD4(+)CD25(-)Foxp3(+) T细胞数量分别与eGFR和血清Cr水平呈正相关。