National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-ecological Process in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125, China.
Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, School of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.
Oxid Med Cell Longev. 2022 Mar 12;2022:6316611. doi: 10.1155/2022/6316611. eCollection 2022.
Chlorogenic acid (CGA), as one of the richest polyphenol compounds in nature, has broad applications in many fields due to its various biological properties. However, initial data on the effects of dietary CGA on protein synthesis and related basal metabolic activity has rarely been reported. The current study is aimed at (1) determining whether dietary CGA supplementation improves the growth performance and carcass traits, (2) assessing whether dietary CGA alters the free amino acid profile, and (3) verifying whether dietary CGA promotes muscle protein synthesis in finishing pigs. Thirty-two (Large × White × Landrace) finishing barrows with an average initial body weight of 71.89 ± 0.92 kg were randomly allotted to 4 groups and fed diets supplemented with 0, 0.02%, 0.04%, and 0.08% CGA, respectively. The results indicated that, compared with the control group, dietary supplementation with 0.04% CGA slightly stimulated the growth performance of pigs, whereas no significant correlation was noted between the dietary CGA levels and animal growth ( > 0.05). Furthermore, the carcass traits of pigs were improved by 0.04% dietary CGA ( < 0.01). In addition, dietary CGA significantly improved the serum free amino acid profiles of pigs ( < 0.01), while 0.04% dietary CGA promoted more amino acids to translocate to skeletal muscles ( < 0.05). The relative mRNA expression levels of SNAT2 in both longissimus dorsi (LD) and biceps femoris (BF) muscles were augmented in the 0.02% and 0.04% groups ( < 0.05), and the LAT1 mRNA expression in the BF muscle was elevated in the 0.02% group ( < 0.05). We also found that dietary CGA supplementation at the levels of 0.04% or 0.08% promoted the expression of p-Akt and activated the mTOR-S6K1-4EBP1 axis in the LD muscle ( < 0.05). Besides, the MAFbx mRNA abundance in the 0.02% and 0.04% groups was significantly lower ( < 0.05). Our results revealed that dietary supplementation with CGA of 0.04% improved the free amino acid profile and enhanced muscle protein biosynthesis in the LD muscle in finishing pigs.
没食子酸（CGA）作为自然界中最丰富的多酚化合物之一，由于其多种生物特性，在许多领域都有广泛的应用。然而，关于膳食 CGA 对蛋白质合成和相关基础代谢活性的影响的初始数据很少有报道。本研究旨在：（1）确定膳食 CGA 补充是否能提高生长性能和胴体性状，（2）评估膳食 CGA 是否改变游离氨基酸谱，（3）验证膳食 CGA 是否能促进育肥猪的肌肉蛋白质合成。32 头（长×白×兰德瑞斯）育肥阉公猪，平均初始体重为 71.89±0.92kg，随机分为 4 组，分别饲喂添加 0、0.02%、0.04%和 0.08% CGA 的日粮。结果表明，与对照组相比，日粮中添加 0.04%CGA 略微刺激了猪的生长性能，而日粮 CGA 水平与动物生长之间没有显著相关性（>0.05）。此外，日粮 CGA 水平提高了 0.04%时改善了猪的胴体性状（<0.01）。此外，膳食 CGA 显著改善了猪的血清游离氨基酸谱（<0.01），同时 0.04%的膳食 CGA 促进了更多的氨基酸向骨骼肌转移（<0.05）。长肌（LD）和股二头肌（BF）肌肉中 SNAT2 的相对 mRNA 表达水平在 0.02%和 0.04%组中均升高（<0.05），BF 肌肉中的 LAT1mRNA 表达在 0.02%组中升高（<0.05）。我们还发现，日粮 CGA 补充 0.04%或 0.08%水平可促进 LD 肌肉中 p-Akt 的表达，并激活 mTOR-S6K1-4EBP1 轴（<0.05）。此外，0.02%和 0.04%组的 MAFbxmRNA 丰度显著降低（<0.05）。我们的结果表明，日粮补充 0.04%的 CGA 可改善育肥猪 LD 肌肉中的游离氨基酸谱，并增强肌肉蛋白质合成。
