Colacchio T A, Memoli V A, Hildebrandt L
Department of Surgery, Dartmouth Medical School, Hanover, NH 03755.
Arch Surg. 1989 Feb;124(2):217-21. doi: 10.1001/archsurg.1989.01410020091014.
The organospecific, 1,2-dimethylhydrazine-induced murine tumor model was used to test the effects on tumor formation of the following dietary supplements: (1) ascorbic acid, 7% per weight; (2) alpha tocopherol, 1% per weight; (3) beta carotene, 1% per weight; and (4) canthazanthin, 1% per weight. Following a four-week dietary acclimation, a 16-week 1,2-dimethylhydrazine induction, and a four-week hiatus, the animals were killed, underwent autopsies, and tumor formation was recorded. The antioxidant supplements of ascorbic acid and alpha tocopherol resulted in a significant decrease in tumor formation when compared with control groups. In contrast, the beta carotene group showed no difference in tumor formation, and canthazanthin, a non-provitamin A carotenoid, resulted in an increase in tumor formation when compared with controls. In addition, the K-gel powder control diet (a carrier medium for alpha tocopherol acetate) had a significantly higher rate of tumor formation than the regular chow and placebo beadlet control diets. In sum, ascorbic acid and alpha tocopherol demonstrated a clear chemopreventive effect, whereas beta carotene had no effect, and canthazanthin appeared to function as a promoter in this organospecific tumor model.
采用器官特异性的、1,2 - 二甲基肼诱导的小鼠肿瘤模型，来测试以下膳食补充剂对肿瘤形成的影响：（1）抗坏血酸，重量占比7%；（2）α - 生育酚，重量占比1%；（3）β - 胡萝卜素，重量占比1%；（4）角黄素，重量占比1%。经过四周的饮食适应期、16周的1,2 - 二甲基肼诱导期以及四周的间歇期后，处死动物，进行解剖，并记录肿瘤形成情况。与对照组相比，抗坏血酸和α - 生育酚这两种抗氧化剂补充剂使肿瘤形成显著减少。相比之下，β - 胡萝卜素组在肿瘤形成方面没有差异，并且与对照组相比，非维生素A类胡萝卜素角黄素导致肿瘤形成增加。此外，K - 凝胶粉对照饮食（α - 生育酚醋酸酯的载体介质）的肿瘤形成率显著高于常规饲料和安慰剂小丸对照饮食。总之，抗坏血酸和α - 生育酚显示出明显的化学预防作用，而β - 胡萝卜素没有作用，并且在这个器官特异性肿瘤模型中角黄素似乎起到了促癌作用。
