Grau Marijke, Friederichs Petra, Krehan Sebastian, Koliamitra Christina, Suhr Frank, Bloch Wilhelm
Clin Hemorheol Microcirc. 2015 Jul 16;60(2):215-29. doi: 10.3233/CH-141850.
During storage, red blood cells (RBC) become more susceptible to hemolysis and it has also been shown that RBC deformability, which is influenced by RBC nitric oxide synthase (RBC-NOS) activity, decreases during blood storage while a correlation between these two parameters under storage conditions has not been investigated so far. Therefore, blood from 15 male volunteers was anticoagulated, leuko-reduced and stored as either concentrated RBC or RBC diluted in saline-adenine-glucose-mannitol (SAGM) for eight weeks at 4°C and results were compared to data obtained from freshly drawn blood. During storage, decrease of RBC deformability was related to increased mean cellular volume and increased cell lysis but also to a decrease in RBC-NOS activation. The changes were more pronounced in concentrated RBC than in RBC diluted in SAGM suggesting that the storage method affects the quality of blood. These data shed new light on mechanisms underlying the phenomenon of storage lesion and reveal that RBC-NOS activation and possibly nitric oxide production in RBC are key elements that are influenced by storage and in turn alter deformability. Further studies should therefore also focus on improving these parameters during storage to improve the quality of stored blood with respect to blood transfusion.
在储存过程中，红细胞（RBC）变得更容易发生溶血，并且也有研究表明，受红细胞一氧化氮合酶（RBC-NOS）活性影响的红细胞变形性在血液储存期间会降低，而到目前为止尚未研究这两个参数在储存条件下的相关性。因此，采集了15名男性志愿者的血液，进行抗凝、白细胞去除处理，然后分别作为浓缩红细胞或用生理盐水-腺嘌呤-葡萄糖-甘露醇（SAGM）稀释的红细胞在4°C下储存8周，并将结果与新鲜采集的血液数据进行比较。在储存期间，红细胞变形性的降低与平均细胞体积增加、细胞裂解增加有关，也与RBC-NOS活性降低有关。这些变化在浓缩红细胞中比在SAGM稀释的红细胞中更明显，表明储存方法会影响血液质量。这些数据为储存损伤现象背后的机制提供了新的线索，并揭示RBC-NOS活性以及红细胞中一氧化氮的产生可能是受储存影响的关键因素，进而改变变形性。因此，进一步的研究也应侧重于在储存期间改善这些参数，以提高用于输血的储存血液的质量。
