Song Huadong, Liu Ying, Zhang Bin, Tian Kangzhen, Zhu Panpan, Lu Hao, Tang Qi
Jiangsu Key Laboratory of Advanced Laser Materials and Devices, College of Physics & Electronic Engineering, Jiangsu Normal University, Xuzhou, Jiangsu, China.
Biomed Opt Express. 2016 Dec 19;8(1):384-394. doi: 10.1364/BOE.8.000384. eCollection 2017 Jan 1.
The elasticity of red cell membrane is a critical physiological index for the activity of RBC. Study of the inherent mechanism for RBCs membrane elasticity transformation is attention-getting all along. This paper proposes an optimized measurement method of erythrocytes membrane shear modulus incorporating acousto-optic deflector (AOD) scanning optical tweezers system. By use of this method, both membrane shear moduli and sizes of RBCs with different in vitro times were determined. The experimental results reveal that the RBCs membrane elasticity and size decline with in vitro time extension. In addition, semi quantitative measurements of S-nitrosothiol content in blood using fluorescent spectrometry during in vitro storage show that RBCs membrane elasticity change is positively associated with the S-nitrosylation level of blood. The analysis considered that the diminished activity of the nitric oxide synthase makes the S-nitrosylation of in vitro blood weaker gradually. The main reason for worse elasticity of the in vitro RBCs is that S-nitrosylation effect of spectrin fades. These results will provide a guideline for further study of in vitro cells activity and other clinical applications.
红细胞膜弹性是反映红细胞活性的关键生理指标。红细胞膜弹性转变的内在机制研究一直备受关注。本文提出了一种结合声光偏转器（AOD）扫描光镊系统的红细胞膜剪切模量优化测量方法。利用该方法测定了不同体外保存时间的红细胞的膜剪切模量和大小。实验结果表明，随着体外保存时间的延长，红细胞膜弹性和大小均下降。此外，体外保存期间采用荧光光谱法对血液中S-亚硝基硫醇含量进行半定量测量，结果显示红细胞膜弹性变化与血液的S-亚硝基化水平呈正相关。分析认为，一氧化氮合酶活性降低使得体外血液的S-亚硝基化作用逐渐减弱。体外红细胞弹性变差的主要原因是血影蛋白的S-亚硝基化作用消退。这些结果将为进一步研究体外细胞活性及其他临床应用提供指导。
