一种从土壤中回收DNA、RNA和蛋白质的顺序共提取方法，用于未来基于系统的方法。
A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches.
作者信息
Gunnigle Eoin, Ramond Jean-Baptiste, Frossard Aline, Seeley Mary, Cowan Don
机构信息
Centre for Microbial Ecology and Genomics (CMEG), Department of Genetics, University of Pretoria, South Africa.
Gobabeb Research and Training Centre, Walvis Bay, Namibia.
出版信息
J Microbiol Methods. 2014 Aug;103:118-23. doi: 10.1016/j.mimet.2014.06.004. Epub 2014 Jun 11.
A co-extraction protocol that sequentially isolates core biopolymer fractions (DNA, RNA, protein) from edaphic microbial communities is presented. In order to confirm compatibility with downstream analyses, bacterial T-RFLP profiles were generated from the DNA- and RNA-derived fractions of an arid-based soil, with metaproteomics undertaken on the corresponding protein fraction.
本文介绍了一种共提取方案，该方案可从土壤微生物群落中依次分离核心生物聚合物组分（DNA、RNA、蛋白质）。为了确认与下游分析的兼容性，从干旱土壤的DNA和RNA衍生组分中生成细菌T-RFLP图谱，并对相应的蛋白质组分进行元蛋白质组学分析。
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