Matthews K A, Miller D F, Kaufman T C
Department of Biology, Indiana University, Bloomington 47405.
Dev Biol. 1989 Mar;132(1):45-61. doi: 10.1016/0012-1606(89)90203-0.
The developmental pattern of gene expression of the Drosophila melanogaster alpha-tubulin family has been examined in detail at both the mRNA and protein levels. Northern data from 16 stages of development have been quantified to produce estimates of pool sizes of each of the alpha-tubulin transcripts through development. The in situ pattern of alpha 67C RNA localization in developing oocytes and early embryos has also been determined. At the protein level, two of the three previously unidentified products of alpha-tubulin genes (alpha 67C and alpha 85E) have been identified. Evidence that protein from the fourth gene comigrates with the ubiquitously expressed alpha 84B is presented. In addition to the primary translational products of the alpha-tubulin genes, an elaborate series of post-translationally modified alpha-tubulins has been resolved. The developmental profiles of both synthesis and accumulation of these alpha-tubulin proteins are described.
黑腹果蝇α-微管蛋白家族基因表达的发育模式已在mRNA和蛋白质水平上进行了详细研究。对来自16个发育阶段的Northern数据进行了定量分析，以估算发育过程中每种α-微管蛋白转录本的库大小。还确定了α 67C RNA在发育中的卵母细胞和早期胚胎中的原位定位模式。在蛋白质水平上，已鉴定出α-微管蛋白基因的三种先前未鉴定产物中的两种（α 67C和α 85E）。提供了来自第四个基因的蛋白质与普遍表达的α 84B共迁移的证据。除了α-微管蛋白基因的初级翻译产物外，还解析出了一系列复杂的翻译后修饰的α-微管蛋白。描述了这些α-微管蛋白的合成和积累的发育概况。
