Division of Plastic and Reconstructive Surgery, Washington University School of Medicine, St. Louis, MO 63110, USA.
Microsurgery. 2012 Jul;32(5):370-6. doi: 10.1002/micr.21967. Epub 2012 Mar 31.
Glial cell line-derived neurotrophic factor (GDNF) has potent axonal growth and survival effects on motoneurons. This study used transgenic Myo-GDNF mice to assess the effects of targeted GDNF overexpression on functional recovery after botulinum toxin type A (BTxA) chemodenervation. BTxA (0.1 U) was injected into the tibialis anterior (TA) muscle of wild-type CF1 and transgenic Myo-GDNF mice. On days 1, 7, 14, and 21 after injection, evoked muscle force production and muscle mass were measured (n = 6, for each group at each time point). Greater maximal tetanic force and calculated specific force were evoked in Myo-GDNF animals when compared with control CF1 animals at days 1, 7, and 21. However, the differences were not statistically significant. Similarly, modest reductions in muscle atrophy in the Myo-GDNF group at all time points were not statistically significant. Targeted overexpression of GDNF in the muscles of Myo-GDNF mice did not improve motor recovery in the first 21 days after BTxA chemodenervation.
胶质细胞源性神经营养因子（GDNF）对运动神经元具有很强的轴突生长和存活作用。本研究使用转基因 Myo-GDNF 小鼠来评估靶向 GDNF 过表达对肉毒杆菌毒素 A（BTxA）化学去神经支配后功能恢复的影响。BTxA（0.1 U）被注射到野生型 CF1 和转基因 Myo-GDNF 小鼠的胫骨前肌（TA）中。在注射后第 1、7、14 和 21 天，测量了诱发的肌肉力量产生和肌肉质量（每组 6 只，每个时间点）。与对照 CF1 动物相比，Myo-GDNF 动物在第 1、7 和 21 天产生了更大的最大强直力和计算出的比力。然而，差异没有统计学意义。同样，Myo-GDNF 组在所有时间点肌肉萎缩的适度减少也没有统计学意义。在 BTxA 化学去神经支配后 21 天内，Myo-GDNF 小鼠肌肉中 GDNF 的靶向过表达并未改善运动恢复。
