Wang Li-Jun, Xiong Juan, Liu Shu-Ting, Liu Xin-Hua, Hu Jin-Feng
Department of Natural Products Chemistry, School of Pharmacy, Fudan University, No. 826 Zhangheng Road, Shanghai 201203, P. R. China, (phone/fax: +86 21 51980172).
Chem Biodivers. 2014 Jun;11(6):919-28. doi: 10.1002/cbdv.201300283.
Five new and seven known mono-sesquiterpenoids (1-5 and 6-12, resp.) together with five known lindenane-type disesquiterpenoids, 13-17, were isolated from the whole plant of Chloranthus henryi. Based on spectroscopic methods, the new structures were established to be (5S,6R,8S,10R)-6-hydroxyeudesma-4(15),7(11)-diene-12,8-olide (1), 6α-hydroxyeudesma-4(15),7(11),8(9)-triene-12,8-olide (2), 8,12-epoxy-1β-hydroxyeudesma-4(15),7,11-trien-6-one (3), 12-oxochloraniolide A (4), and (4α)-8-hydroxy-12-norcardina-6,8,10-trien-11-one (5), respectively. Among the isolates, compound 2, zederone epoxide (8), spicachlorantin G (13), chloramultilide A (14), shizukaol B (15), and spicachlorantin B (17) showed significant anti-neuroinflammatory effects by inhibiting nitric-oxide (NO) production in lipopolysaccharide (LPS)-stimulated murine BV-2 microglial cells with relatively low cytotoxicity.
从宽叶金粟兰全株中分离得到5个新的和7个已知的单萜-倍半萜类化合物（分别为1-5和6-12），以及5个已知的椴烷型双倍半萜类化合物13-17。基于光谱方法，确定新结构分别为(5S,6R,8S,10R)-6-羟基桉叶-4(15),7(11)-二烯-12,8-内酯（1）、6α-羟基桉叶-4(15),7(11),8(9)-三烯-12,8-内酯（2）、8,12-环氧-1β-羟基桉叶-4(15),7,11-三烯-6-酮（3）、12-氧代金粟兰内酯A（4）和(4α)-8-羟基-12-降卡迪那-6,8,10-三烯-11-酮（5）。在分离得到的化合物中，化合物2、氧化泽德酮（8）、穗花金粟兰素G（13）、多花金粟兰内酯A（14）、静香醇B（15）和穗花金粟兰素B（17）在脂多糖（LPS）刺激的小鼠BV-2小胶质细胞中通过抑制一氧化氮（NO）的产生表现出显著的抗神经炎症作用，且细胞毒性相对较低。
