Búfalo Michelle Cristiane, Bordon-Graciani Ana Paula, Conti Bruno José, de Assis Golim Marjorie, Sforcin José Maurício
Department of Microbiology and Immunology, Biosciences Institute, UNESP, Botucatu, SP, Brazil.
J Pharm Pharmacol. 2014 Oct;66(10):1497-504. doi: 10.1111/jphp.12279. Epub 2014 Jun 16.
Propolis is a beehive product and its immunomodulatory action has been documented; however, little is known concerning its mechanisms of action on human cells. Propolis influence on the initial events of the immune response was assessed, evaluating cell markers, cytokine production and the fungicidal activity of human monocytes.
Toll-like receptor (TLR)-2, TLR-4, human leukocyte antigen-DR and cluster of differentiation (CD)80 expression by human monocytes was assessed using a FACSCalibur flow cytometer, cytokine production (tumour necrosis factor (TNF)-α and interleukin (IL)-10) was determined by ELISA and the candidacidal activity was investigated after monocytes incubation with propolis and challenged with Candida albicans. The role of TLR-2 and TLR-4 on propolis action was assessed as well.
Propolis upregulated TLR-4 and CD80 expression and affected TNF-α and IL-10 production, depending on concentration. Propolis also increased the fungicidal activity of monocytes. Cytokine production was decreased by blocking TLR-4, whereas the fungicidal activity was affected by blocking TLR-2.
Propolis exerted an immunomodulatory action on cell receptors, cytokine production and fungicidal activity of human monocytes without affecting cell viability and depending on concentration. TLR-2 and TLR-4 may be involved in its mechanism of action.
蜂胶是一种蜂巢产品，其免疫调节作用已有文献记载；然而，关于其对人类细胞的作用机制却知之甚少。评估了蜂胶对免疫反应初始事件的影响，检测了细胞标志物、细胞因子产生以及人类单核细胞的杀真菌活性。
使用FACSCalibur流式细胞仪评估人类单核细胞中Toll样受体（TLR）-2、TLR-4、人类白细胞抗原-DR和分化簇（CD）80的表达，通过酶联免疫吸附测定法（ELISA）测定细胞因子产生（肿瘤坏死因子（TNF）-α和白细胞介素（IL）-10），并在单核细胞与蜂胶孵育后用白色念珠菌攻击，研究其杀念珠菌活性。还评估了TLR-2和TLR-4在蜂胶作用中的作用。
蜂胶上调TLR-4和CD80表达，并根据浓度影响TNF-α和IL-10的产生。蜂胶还增强了单核细胞的杀真菌活性。阻断TLR-4可降低细胞因子产生，而阻断TLR-2则影响杀真菌活性。
蜂胶对人类单核细胞的细胞受体、细胞因子产生和杀真菌活性具有免疫调节作用，且不影响细胞活力，并取决于浓度。TLR-2和TLR-4可能参与其作用机制。
