Department of Microbiology and Immunology, Biosciences Institute, Sao Paulo State University, Botucatu, Sao Paulo, Brazil.
Nat Prod Res. 2012;26(5):446-53. doi: 10.1080/14786419.2010.482049. Epub 2011 Jun 24.
Our group has been investigating the immunomodulatory activity of propolis in stressed mice. In this work, we wish to report the action of propolis in chronically stressed mice, assessing the Toll-like receptor (TLR-2 and TLR-4) expression by spleen cells and corticosterone levels as a stress indicator. Male C57BL/6 mice were divided into four groups: G1 was considered the control; G2 was treated with propolis (200 mg kg(-1)); G3 was submitted to restraint stress for 14 days; and G4 was treated with propolis and immediately submitted to stress. After sacrifice, spleens were removed and TLR-2 and TLR-4 gene expression was analysed. TLR-2 and TLR-4 expression was increased in propolis-treated mice, and propolis administration to stressed mice prevented the inhibition of TLR-2 and TLR-4 expression. No differences were seen in the corticosterone levels among the groups. Propolis exerted an immunomodulatory action in chronically stressed mice, upregulating TLR-2 and TLR-4 mRNA expression, contributing to the recognition of microorganisms and favouring the initial steps of the immune response during stress.
我们的小组一直在研究蜂胶对压力小鼠的免疫调节作用。在这项工作中，我们希望报告蜂胶对慢性应激小鼠的作用，评估脾细胞中的 Toll 样受体（TLR-2 和 TLR-4）表达和皮质酮水平作为应激指标。雄性 C57BL/6 小鼠分为四组：G1 为对照组；G2 用蜂胶（200mg/kg）处理；G3 接受 14 天的束缚应激；G4 用蜂胶处理并立即进行应激。处死小鼠后，取出脾脏，分析 TLR-2 和 TLR-4 基因表达。蜂胶处理组的 TLR-2 和 TLR-4 表达增加，给予应激小鼠蜂胶可防止 TLR-2 和 TLR-4 表达抑制。各组之间的皮质酮水平没有差异。蜂胶对慢性应激小鼠具有免疫调节作用，上调 TLR-2 和 TLR-4 mRNA 表达，有助于在应激期间识别微生物并促进免疫反应的初始步骤。
