利用鼠尾藻生物合成金纳米颗粒及其对HeLa细胞的细胞毒性作用。
Biosynthesis of gold nanoparticles using Sargassum swartzii and its cytotoxicity effect on HeLa cells.
作者信息
Dhas T Stalin, Kumar V Ganesh, Karthick V, Govindaraju K, Shankara Narayana T
机构信息
Nanoscience Division, Centre for Ocean Research, Sathyabama University, Chennai 600 119, India.
Nanoscience Division, Centre for Ocean Research, Sathyabama University, Chennai 600 119, India.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2014 Dec 10;133:102-6. doi: 10.1016/j.saa.2014.05.042. Epub 2014 May 27.
In this investigation, biological synthesis of gold nanoparticles (AuNPs) using Sargassum swartzii and its cytotoxicity against human cervical carcinoma (HeLa) cells is reported. The biological synthesis involved the reduction of chloroauric acid led to the formation of AuNPs within 5min at 60°C and the formation of AuNPs was confirmed using UV-vis spectrophotometer. The AuNPs were stable; spherical in shape with well-defined dimensions, and the average size of the particle is 35nm. A zeta potential value of -27.6mV revealed synthesized AuNPs were highly stable. The synthesized AuNPs exhibited a dose-dependent cytotoxicity against human cervical carcinoma (HeLa) cells. Furthermore, induction of apoptosis was measured by DAPI (4',6-Diamidino-2-phenylindole dihydrochloride) staining.
在本研究中，报道了利用鼠尾藻进行金纳米颗粒（AuNPs）的生物合成及其对人子宫颈癌（HeLa）细胞的细胞毒性。生物合成过程涉及氯金酸的还原，在60℃下5分钟内导致AuNPs形成，并用紫外可见分光光度计确认了AuNPs的形成。AuNPs稳定；呈球形，尺寸明确，颗粒平均大小为35nm。ζ电位值为-27.6mV表明合成的AuNPs高度稳定。合成的AuNPs对人子宫颈癌（HeLa）细胞表现出剂量依赖性细胞毒性。此外，通过4',6-二脒基-2-苯基吲哚二盐酸盐（DAPI）染色来检测细胞凋亡的诱导情况。
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