a Department of Oncology, Zhengzhou Central Hospital Affiliated To Zhengzhou University , Zhengzhou City , China.
b Department of Biology, College of Science, Al-Zulfi, Majmaah University , Majmaah , Kingdom of Saudi Arabia.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):1938-1946. doi: 10.1080/21691401.2019.1614017.
Bionanotechnology has pivotal role in the development of a novel therapy, applications of gold nanoparticles (AuNPs) in the treatment of cancer. In this study, we found that therapeutics, pharmaceutics and diagnostic effectiveness of photosynthesized Catharanthus roseus (CR) AuNPs induces mitochondrial-mediated apoptotic signalling pathways via reactive oxygen species (ROS) induced cytotoxicity in cervical cancer cell line (HeLa) by in vitro model. The present examinations were for the most part centred around the gold chloride and photosynthesis AuNPs from the fluid leaf concentrate of CR and their harmful impacts on HeLa cell lines. The synthesized AuNPs were characterized using numerous biophysical analyses such as UV-vis, DLS, EDX, HR-TEM, SAED, FTIR and AFM. The synthesized AuNPs in the particle size range of 25-35 nm was confirmed by HR-TEM. The element gold and the crystalline nature of AuNPs were finalized using EDX, respectively. Anticancer potential of CR-AuNPs was studied using HeLa cells and the cytotoxic mechanism has been evaluated using MTT, mitochondrial-mediated apoptotic pathway through AO/EtBr staining assay, pro-apoptotic (Bax), anti-apoptotic (Bcl-2 and Bid) protein expression western blotting analysis and caspases activity using ELISA analysis. In in vitro study, the IC50 of HeLa cells was found to be 5 µg/ml confirmed using MTT assay. The present data revealed that drug delivery vehicles developed on CR-AuNPs nanocomplexes might include extensive purpose in human cancer diagnosis and treatment.
生物纳米技术在新型疗法的发展中起着关键作用，金纳米粒子（AuNPs）在癌症治疗中的应用就是一个例子。在这项研究中，我们发现，通过体外模型，合成的长春花（CR）AuNPs 通过活性氧（ROS）诱导的细胞毒性诱导线粒体介导的凋亡信号通路，具有治疗、药剂学和诊断效果，可用于宫颈癌（HeLa）细胞系。目前的检查主要集中在金氯化物和 CR 液体叶提取物的光合作用 AuNPs 及其对 HeLa 细胞系的有害影响上。合成的 AuNPs 通过多种生物物理分析进行了表征，如 UV-vis、DLS、EDX、HR-TEM、SAED、FTIR 和 AFM。通过 HR-TEM 证实了 AuNPs 的粒径范围在 25-35nm 之间。使用 EDX 分别确定了元素金和 AuNPs 的晶体性质。使用 HeLa 细胞研究了长春花 AuNPs 的抗癌潜力，并通过 MTT、AO/EtBr 染色试验评估线粒体介导的凋亡途径、促凋亡（Bax）、抗凋亡（Bcl-2 和 Bid）蛋白表达 Western blot 分析和 caspase 活性使用 ELISA 分析评估了细胞毒性机制。在体外研究中，通过 MTT 测定发现 HeLa 细胞的 IC50 为 5µg/ml。目前的数据表明，基于 CR-AuNPs 纳米复合物开发的药物递送载体可能在人类癌症的诊断和治疗中有广泛的用途。
