Furuya T, Ochi H, Watanabe S
Epidemiology Division, National Cancer Center Research Institute, Tokyo.
Jpn J Clin Oncol. 1989 Mar;19(1):23-5.
The relation between the expression of common fragile sites and chromosomal breakpoints in neoplastic cells in the same patients has not been well studied. In the present study, the frequency and distribution of aphidicolin-induced breaks on chromosomes 8 and 21 in peripheral blood lymphocytes (PBL) taken from three patients with acute myeloid leukemia, French-American-British classification type M2, having chromosomal translocation t(8;21)(q22;q22) (M2t) were studied prior to any initial treatment. Seven patients with other types of acute leukemia without t(8;21) and 13 healthy people were also studied. In PBL from patients with M2t, the numbers of chromosomal breaks were 1, 7 and 3/216 metaphasees at bands 8q21, 8q22 and 8q24, respectively; the frequencies at 8q22 and 8q24 being significantly higher than those for patients with the other leukemias and for the healthy subjects (p less than 0.001 and p less than 0.01, respectively). These results suggest that the fragility on chromosome 8q21-24 is related to a predisposition to this particular type of leukemia.
同一患者肿瘤细胞中常见脆性位点的表达与染色体断点之间的关系尚未得到充分研究。在本研究中，对3例法国-美国-英国协作组分类为M2型急性髓细胞白血病且有染色体易位t(8;21)(q22;q22)（M2t）的患者，在任何初始治疗之前，研究了从其外周血淋巴细胞（PBL）中由阿非科林诱导的8号和21号染色体上的断裂频率和分布。还研究了7例无t(8;21)的其他类型急性白血病患者和13名健康人。在M2t患者的PBL中，8q21、8q22和8q24带处染色体断裂数分别为1、7和3/216个中期；8q22和8q24处的频率显著高于其他白血病患者和健康受试者（分别为p<0.001和p<0.01）。这些结果表明，8q21 - 24染色体上的脆性与这种特定类型白血病的易感性有关。
