Mishra A K, Dufour H, Roche P-H, Lonjon M, Raoult D, Fournier P-E
URMITE, UM63, CNRS 7278, IRD 198, Inserm 1095, Aix-Marseille Université, Faculté de Médecine, 27 Boulevard Jean Moulin, 13385, Marseille cedex 05, France.
Eur J Clin Microbiol Infect Dis. 2014 Dec;33(12):2083-93. doi: 10.1007/s10096-014-2166-z. Epub 2014 Jun 18.
A brain abscess is a life-threatening infection, frequently with serious sequelae. Culture-based methods present many limitations and do not enable an exhaustive documentation of the bacterial flora. 16S rRNA-based amplification, cloning, and high-throughput sequencing have dramatically increased the number of identified agents of brain abscesses, showing that the causative flora is polymicrobial in up to 40 % of cases, with the presence of at least one anaerobic bacterium. In contrast, real-time polymerase chain reaction (PCR) technology is an appealing alternative to culture-based methods for diagnosing brain abscesses due to its speed, sensitivity, and specificity. Molecular typing is available for several bacterial and fungal genera, and this user-friendly tool is accessible for the clinical microbiology laboratory to diagnose microbes involved in a brain abscess. This article reviews the applications of the currently available tools for the etiological diagnosis of a brain abscess.
脑脓肿是一种危及生命的感染，常伴有严重的后遗症。基于培养的方法存在许多局限性，无法全面记录细菌菌群。基于16S rRNA的扩增、克隆和高通量测序极大地增加了已鉴定的脑脓肿病原体数量，表明在高达40%的病例中，致病菌群是多微生物的，且至少存在一种厌氧菌。相比之下，实时聚合酶链反应（PCR）技术因其速度、灵敏度和特异性，是一种比基于培养的方法更具吸引力的诊断脑脓肿的替代方法。几种细菌和真菌属均可进行分子分型，临床微生物实验室可以使用这种用户友好型工具来诊断参与脑脓肿的微生物。本文综述了目前可用工具在脑脓肿病因诊断中的应用。
