Liu Lu, Zhang Ying, Gu He-feng, Zhang Kai-qiang, Ma Lin, Cheng Rui-bo, Zhang Si-yu
Department of Preventive Dentistry, School of Stomatology, China Medical University, Liaoning Institute of Dental Research. Shenyang 110002, Liaoning Province, China.E-mail:
Shanghai Kou Qiang Yi Xue. 2014 Apr;23(2):129-32.
To investigate the effect of fluoride on the metabolism of teeth and bone in rats, and to probe the mechanism of pathogenesis of dental fluorosis.
A total of 48 Wistar rats were randomly and equally divided into 4 groups including control group (distilled water), low-dose group(NaF,50 mg/L), medium-dose group (NaF,100 mg/L) and high-dose group (NaF, 150 mg/L). After 8 weeks, the rats were sacrificed under anesthesia, and serums were collected. The biochemical technique was used to test serum calcium. Changes in the fluorine content in serums and teeth of each group were analyzed with fluoride ion selective electrode method. Radioimmunoassay was employed to detect the levels of osteocalcin (OC), parathormone (PTH) and calcitonin (CT), respectively. SPSS13.0 software package was used for statistical analysis.
The fluorine content in serum and teeth in the fluoride group were significantly higher than that of the control group (P<0.05), and increased with the increasing concentrations (F value was 11.234 and 275.148 respectively, P<0.01). The level of calcium in serum (F=3.906, P<0.05) in the fluoride group was significantly lower than in the control group. The level of PTH and OC in serum in medium and high-dose group were significantly higher than in the control group (P<0.01), with the level of CT in high-dose group decreased significantly (P<0.01). The differences of the level of OC, PTH, CT between groups were significant (F value was 8.548, 3.801 and 5.121 respectively, P<0.05).
Fluoride affects the metabolism of teeth and bone in rats and OC, PTH, CT plays a key role in the pathogenesis of dental fluorosis.
探讨氟对大鼠牙齿和骨骼代谢的影响，并探究氟斑牙发病机制。
将48只Wistar大鼠随机均分为4组，即对照组（蒸馏水）、低剂量组（NaF，50 mg/L）、中剂量组（NaF，100 mg/L）和高剂量组（NaF，150 mg/L）。8周后，将大鼠麻醉处死并采集血清。采用生化技术检测血清钙。用氟离子选择性电极法分析各组血清和牙齿中氟含量的变化。分别采用放射免疫分析法检测骨钙素（OC）、甲状旁腺激素（PTH）和降钙素（CT）水平。使用SPSS13.0软件包进行统计分析。
氟组血清和牙齿中的氟含量显著高于对照组（P<0.05），且随浓度升高而增加（F值分别为11.234和275.148，P<0.01）。氟组血清钙水平（F=3.906，P<0.05）显著低于对照组。中、高剂量组血清中PTH和OC水平显著高于对照组（P<0.01），高剂量组CT水平显著降低（P<0.01）。各组间OC、PTH、CT水平差异有统计学意义（F值分别为8.548、3.801和5.121，P<0.05）。
氟影响大鼠牙齿和骨骼代谢，OC、PTH、CT在氟斑牙发病机制中起关键作用。
