Asai Germanium Research Institute Co., Ltd., 3-131 Suzuranoka, Hakodate, Hokkaido 042-0958, Japan.
Nippon Beet Sugar Mfg. Co., Ltd., Nishi 13, Minami 9 Sen, Inada, Obihiro, Hokkaido 080-0831, Japan.
Biosci Microbiota Food Health. 2012;31(2):37-45. doi: 10.12938/bmfh.31.37. Epub 2012 Apr 20.
Poly-trans-[(2-carboxyethyl) germasesquioxane] (Ge-132) is the most common organic germanium compound. The ingestion of Ge-132 promotes bile secretion. We assessed the rat caecal characteristics after the administration of Ge-132 and raffinose, a prebiotic oligosaccharide, because both Ge-132 and some prebiotics can change the fecal color to yellow. We also compared the changes in the caecal flora caused by the two compounds. In addition, we evaluated the simultaneous administration of Ge-132 and raffinose and their effects on β-glucuronidase activity, which is known to be a factor related to colon cancer. Male Wistar rats (three weeks old) were given one of the following diets: 1) a control diet (control group), 2) a diet containing 0.05% Ge-132 (Ge-132 group), 3) a diet containing 5% raffinose (RAF group) or 4) a diet containing 0.05% Ge-132 + 5% raffinose (GeRAF group). The Bifidobacterium, Lactobacillus and total bacteria counts were significantly increased by the dietary raffinose, and Ge-132 did not suppress this increase. The raffinose intake increased caecal acetic acid production significantly. The activity of β-glucuronidase in the caecal contents was increased by dietary Ge-132, whereas dietary raffinose decreased the β-glucuronidase activity significantly. These results indicate that the simultaneous intake of dietary raffinose and Ge-132 does not inhibit the effects of either compound on intestinal fermentation and bile secretion. Additionally, the simultaneous intake of both raffinose and Ge-132 could abrogate the increase in β-glucuronidase activity induced by Ge-132 alone.
聚-[（2-羧乙基）锗倍半氧烷]（Ge-132）是最常见的有机锗化合物。摄入 Ge-132 可促进胆汁分泌。我们评估了 Ge-132 和低聚果糖（一种益生元寡糖）给药后大鼠盲肠的特征，因为 Ge-132 和一些益生元都可以使粪便颜色变黄。我们还比较了这两种化合物引起的盲肠菌群变化。此外，我们评估了 Ge-132 和低聚果糖同时给药及其对β-葡萄糖醛酸酶活性的影响，β-葡萄糖醛酸酶活性是与结肠癌相关的一个因素。雄性 Wistar 大鼠（3 周龄）给予以下饮食之一：1）对照饮食（对照组），2）含 0.05%Ge-132 的饮食（Ge-132 组），3）含 5%低聚果糖的饮食（RAF 组）或 4）含 0.05%Ge-132+5%低聚果糖的饮食（GeRAF 组）。低聚果糖喂养显著增加双歧杆菌、乳酸杆菌和总细菌计数，而 Ge-132 并未抑制这种增加。低聚果糖摄入显著增加盲肠乙酸生成。膳食 Ge-132 增加盲肠内容物中β-葡萄糖醛酸酶的活性，而膳食低聚果糖显著降低β-葡萄糖醛酸酶活性。这些结果表明，同时摄入膳食低聚果糖和 Ge-132 不会抑制两种化合物对肠道发酵和胆汁分泌的影响。此外，同时摄入低聚果糖和 Ge-132 可以消除 Ge-132 单独摄入引起的β-葡萄糖醛酸酶活性增加。
