Liu Yen-Wenn, Liao Tan-Wei, Chen Yu-Han, Chiang Yi-Chin, Tsai Ying-Chieh
Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan, ROC.
PLoS One. 2014 Jun 17;9(6):e100105. doi: 10.1371/journal.pone.0100105. eCollection 2014.
This study aimed to investigate the anti-allergic effects of Lactobacillus plantarum K37 (K37) on airway hyperresponsiveness (AHR) and systemic allergic responses in ovalbumin (OVA)-sensitized and -challenged BALB/c mice. Heat-inactivated K37 (105, 107, and 109 CFU/mouse, day) were orally administered to OVA-sensitized BALB/c mice to investigate their effects on AHR, immunoglobulin (Ig) and cytokine production. The results showed that K37 dose-dependently lowered the serum levels of IgE, OVA-specific IgE and OVA-specific IgG1, ameliorated AHR induced by methacholine and suppressed eosinophil infiltration in bronchoalveolar lavage fluid (BALF). The cytokine production in spleen cells culture and BALF showed that K37 drove the immune responses toward T-helper cell type 1 (Th1) responses, elevated levels of IL-2 and IFN-γ, and reduced of IL-4, IL-5 and IL-13. K37 also improved cell infiltration in lung sections. Our results demonstrated that oral administration of K37 alleviated effectively the allergic responses in vivo. Thus, K37 can be a good source material and a promising candidate for prophylactic and therapeutic treatments of allergic diseases, like asthma.
本研究旨在探讨植物乳杆菌K37（K37）对卵清蛋白（OVA）致敏和激发的BALB/c小鼠气道高反应性（AHR）和全身过敏反应的抗过敏作用。将热灭活的K37（105、107和109 CFU/小鼠，每天）口服给予OVA致敏的BALB/c小鼠，以研究其对AHR、免疫球蛋白（Ig）和细胞因子产生的影响。结果表明，K37剂量依赖性地降低了血清IgE、OVA特异性IgE和OVA特异性IgG1水平，改善了乙酰甲胆碱诱导的AHR，并抑制了支气管肺泡灌洗液（BALF）中的嗜酸性粒细胞浸润。脾细胞培养和BALF中的细胞因子产生表明，K37促使免疫反应向1型辅助性T细胞（Th1）反应方向发展，提高了IL-2和IFN-γ水平，并降低了IL-4、IL-5和IL-13水平。K37还改善了肺切片中的细胞浸润。我们的结果表明，口服K37可有效减轻体内的过敏反应。因此，K37可以作为一种良好的原料，是预防和治疗过敏性疾病（如哮喘）的有前途的候选药物。
