Division of Life Science, State Key Laboratory of Marine Pollution, Hong Kong University of Science and Technology (HKUST) , Clearwater Bay, Kowloon, Hong Kong, China.
Environ Sci Technol. 2014 Jul 15;48(14):8152-61. doi: 10.1021/es500655z. Epub 2014 Jun 27.
Concerns for the potential risks of silver nanoparticles (AgNPs) to aquatic organisms have increased. The present study investigated the trophic transfer of AgNPs from brine shrimp (Artemia salina) nauplii to marine medaka. We found that the aggregated AgNPs (20 and 80 nm) and well dispersed 80-nm AgNPs (stabilized by 20 μM Tween 20) could be readily accumulated by brine shrimp, while far less well-dispersed 20-nm AgNPs were accumulated. The assimilation efficiency (AE) of AgNPs in medaka fed AgNPs-contaminated brine shrimp was low (<6%), resulting in a low trophic transfer efficiency (0.01-0.04) after 28 days of chronic dietary exposure. However, such low bioavailability of dietary AgNPs could inhibit the whole-body Na+/K+-ATPase and superoxide dismutase (SOD) activity in the fish within the first 2 weeks of exposure. Significant (p<0.05, two-way ANOVA) inhibition occurred in the high AgNPs-contaminated brine shrimp treatment over 28 days of chronic exposure. Furthermore, reduced growth and water content percentage were also observed in fish fed high dosages of AgNPs-contaminated brine shrimp. Our study highlighted the potential of trophically available AgNPs in bringing toxicity to the marine fish.
人们越来越关注银纳米粒子（AgNPs）对水生生物的潜在风险。本研究调查了从卤虫（Artemia salina）无节幼体到海水鲈的 AgNPs 的营养传递。我们发现，聚集的 AgNPs（20 和 80nm）和分散良好的 80nm AgNPs（由 20μM Tween 20 稳定）可以被卤虫轻易积累，而分散较差的 20nm AgNPs 则积累较少。在慢性饮食暴露 28 天后，食用被 AgNPs 污染的卤虫的海水鲈的 AgNPs 同化效率（AE）较低（<6%），导致营养传递效率（0.01-0.04）较低。然而，饮食中 AgNPs 的这种低生物利用度可能会在暴露的前两周内抑制鱼类体内的全身 Na+/K+-ATPase 和超氧化物歧化酶（SOD）活性。在 28 天的慢性暴露中，高浓度 AgNPs 污染的卤虫处理组发生了显著（p<0.05，双向方差分析）抑制。此外，还观察到食用高剂量 AgNPs 污染卤虫的鱼类生长和含水量百分比降低。我们的研究强调了可营养传递的 AgNPs 对海洋鱼类带来毒性的潜力。
