Chen Shi Xi, Yang Xiao Zhen, Deng Ying, Huang Jing, Li Yan, Sun Qian, Yu Chang-Ping, Zhu Yong, Hong Wan Shu
State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, PR China; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen University, Xiamen, 361102, PR China; State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen University, Xiamen, 361102, PR China.
State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, PR China.
Chemosphere. 2017 Mar;170:51-60. doi: 10.1016/j.chemosphere.2016.12.016. Epub 2016 Dec 7.
Public concern regarding silver nanoparticles (AgNPs) in the environment has been increasing since they can cause adverse effects in some aquatic species. However, few data are actually available on the effects of AgNPs on the germ cells. In the present study, we used the zebrafish ovarian follicle as a model to assess the potentially adverse effects of AgNPs on oocyte maturation (germinal vesicle breakdown, GVBD) in vitro. Similar to the maturation inducing hormone (17α, 20β-dihydroxy-4-pregnen-3-one), AgNPs induced GVBD, and reduced the total cyclic adenosine monophosphate (cAMP) concentration in zebrafish ovarian follicles. The results from transmission electron microscope observation and Hoechst 33342 staining clearly indicated that AgNPs induced apoptosis in ovarian follicle cells surrounding the oocyte. Similar to AgNPs, AgNO also induced GVBD, decreased cAMP concentration and induced apoptosis of ovarian follicle cells. However, the results from gene expression analysis showed that transcript levels of oxidative stress related genes were more sensitive to AgNPs than AgNO. Further more, HO has an ability to induce zebrafish oocytes maturation by induction of apoptosis in ovarian follicle cells. Taken together, the results from our study indicated that oxidative stress appeared to be one of important mechanisms in AgNP induced apoptosis in ovarian follicle cells, which further triggered the GVBD.
自银纳米颗粒（AgNPs）能对某些水生物种产生不利影响以来，公众对环境中银纳米颗粒的关注度一直在上升。然而，关于银纳米颗粒对生殖细胞影响的实际数据却很少。在本研究中，我们以斑马鱼卵巢卵泡为模型，评估银纳米颗粒在体外对卵母细胞成熟（生发泡破裂，GVBD）的潜在不利影响。与成熟诱导激素（17α，20β - 二羟基 - 4 - 孕烯 - 3 - 酮）相似，银纳米颗粒诱导了生发泡破裂，并降低了斑马鱼卵巢卵泡中总环磷酸腺苷（cAMP）的浓度。透射电子显微镜观察和Hoechst 33342染色结果清楚地表明，银纳米颗粒诱导了卵母细胞周围卵巢卵泡细胞的凋亡。与银纳米颗粒相似，硝酸银（AgNO）也诱导了生发泡破裂，降低了cAMP浓度，并诱导了卵巢卵泡细胞的凋亡。然而，基因表达分析结果表明，氧化应激相关基因的转录水平对银纳米颗粒比硝酸银更敏感。此外，过氧化氢（HO）有能力通过诱导卵巢卵泡细胞凋亡来诱导斑马鱼卵母细胞成熟。综上所述，我们的研究结果表明，氧化应激似乎是银纳米颗粒诱导卵巢卵泡细胞凋亡的重要机制之一，这进一步引发了生发泡破裂。
