Xu Chen, Ma Xia, Chen Shiwen, Tao Meifeng, Yuan Lutao, Jing Yao
Department of Neurosurgery, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Yishan Road 600, Shanghai 200233, China.
School of Perfume and Aroma Technology, Shanghai Institute of Technology, Haiquan Road 100, Shanghai 201418, China.
Int J Mol Sci. 2014 Jun 16;15(6):10855-67. doi: 10.3390/ijms150610855.
To improve the efficacy and safety of dural repair in neurosurgical procedures, a new dural material derived from bacterial cellulose (BC) was evaluated in a rabbit model with dural defects. We prepared artificial dura mater using bacterial cellulose which was incubated and fermented from Acetobacter xylinum. The dural defects of the rabbit model were repaired with BC membranes. All surgeries were performed under sodium pentobarbital anesthesia, and all efforts were made to minimize suffering. All animals were humanely euthanized by intravenous injection of phenobarbitone, at each time point, after the operation. Then, the histocompatibility and inflammatory effects of BC were examined by histological examination, real-time fluorescent quantitative polymerase chain reaction (PCR) and Western Blot. BC membranes evenly covered the surface of brain without adhesion. There were seldom inflammatory cells surrounding the membrane during the early postoperative period. The expression of inflammatory cytokines IL-1β, IL-6 and TNF-α as well as iNOS and COX-2 were lower in the BC group compared to the control group at 7, 14 and 21 days after implantation. BC can repair dural defects in rabbit and has a decreased inflammatory response compared to traditional materials. However, the long-term effects need to be validated in larger animals.
为提高神经外科手术中硬脑膜修复的疗效和安全性，在兔硬脑膜缺损模型中对一种源自细菌纤维素（BC）的新型硬脑膜材料进行了评估。我们使用由木醋杆菌培养发酵得到的细菌纤维素制备了人工硬脑膜。兔模型的硬脑膜缺损用BC膜进行修复。所有手术均在戊巴比妥钠麻醉下进行，并尽一切努力将痛苦降至最低。术后每个时间点，所有动物均通过静脉注射苯巴比妥进行人道安乐死。然后，通过组织学检查、实时荧光定量聚合酶链反应（PCR）和蛋白质免疫印迹法检测BC的组织相容性和炎症反应。BC膜均匀覆盖脑表面且无粘连。术后早期膜周围很少有炎性细胞。植入后7、14和21天，BC组炎性细胞因子IL-1β、IL-6和TNF-α以及诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的表达低于对照组。BC可修复兔的硬脑膜缺损，与传统材料相比炎症反应降低。然而，长期效果需要在更大的动物中进行验证。
