Department of Biomedical Science and Research Institute for Bioscience & Biotechnology, Hallym University, Chuncheon, Korea.
J Neurosurg. 2011 Feb;114(2):485-90. doi: 10.3171/2010.9.JNS091764. Epub 2010 Oct 29.
To improve the safety of dura repair in neurosurgical procedures, a new dural material derived from silk fibroin was evaluated in a rat model with a dura mater injury.
The authors prepared new, transparent, artificial dura mater material using silk fibroin from the silkworm, Bombyx mori. The cytotoxic and antiinflammatory effects of the artificial dura mater were examined in vitro and in vivo by histological examination, western blotting, and reverse transcription polymerase chain reaction analyses.
The novel artificial dura mater was not cytotoxic. However, it efficiently reduced cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase expression as well as the expression of the proinflammatory cytokines IL-1β, IL-6, and tumor necrosis factor-α. Cerebrospinal fluid leakage did not occur after repair of the brain of craniotomized rats with the artificial dura mater material.
The new artificial dura mater described in this study appears to be safe for application in neurosurgical procedures and can efficiently inhibit inflammation without side effects or CSF leakage. Although the long-term effects of this artificial dura mater material need to be validated in larger animals, the results from this study indicate that it is suitable for application in neurosurgery.
为提高神经外科手术中硬脑膜修复的安全性，作者在大鼠硬脑膜损伤模型中评估了一种新的源自丝素蛋白的硬脑膜材料。
作者使用家蚕（Bombyx mori）丝素蛋白制备了新型透明人工硬脑膜材料。通过组织学检查、Western blot 和逆转录聚合酶链反应分析，在体外和体内研究了人工硬脑膜的细胞毒性和抗炎作用。
新型人工硬脑膜无细胞毒性，但能有效降低环氧化酶-2（COX-2）和诱导型一氧化氮合酶的表达，以及促炎细胞因子 IL-1β、IL-6 和肿瘤坏死因子-α的表达。用人工硬脑膜材料修复开颅大鼠的脑组织后，未发生脑脊液漏。
本研究中描述的新型人工硬脑膜在神经外科手术中应用似乎是安全的，能有效抑制炎症而无副作用或脑脊液漏。尽管这种人工硬脑膜材料的长期效果需要在更大的动物中验证，但本研究结果表明其适用于神经外科应用。
