Pimentel Camila Bianco, Moraes Aparecida Machado de, Cintra Maria Letícia
aff01.
Universidade Estadual de Campinas, Campinas, SP, Brazil.
An Bras Dermatol. 2014 May-Jun;89(3):410-3. doi: 10.1590/abd1806-4841.20142249.
Cryosurgery is an efficient therapeutic technique used to treat benign and malignant cutaneous diseases. The primary active mechanism of cryosurgery is related to vascular effects on treated tissue. After a cryosurgical procedure, exuberant granulation tissue is formed at the injection site, probably as a result of angiogenic stimulation of the cryogen and inflammatory response, particularly in endothelial cells.
To evaluate the angiogenic effects of freezing, as part of the phenomenon of healing rat skin subjected to previous injury.
Two incisions were made in each of the twenty rats, which were divided randomly into two groups of ten. After 3 days, cryosurgery with liquid nitrogen was performed in one of incisions. The rats' samples were then collected, cut and stained to conduct histopathological examination, to assess the local angiogenesis in differing moments and situations.
It was possible to demonstrate that cryosurgery, in spite of promoting cell death and accentuated local inflammation soon after its application, induces quicker cell proliferation in the affected tissue and maintenance of this rate in a second phase, than in tissue healing without this procedure.
These findings, together with the knowledge that there is a direct relationship between mononuclear cells and neovascularization (the development of a rich system of new vessels in injury caused by cold), suggest that cryosurgery possesses angiogenic stimulus, even though complete healing takes longer to occur. The significance level for statistical tests was 5% (p<0,05).
冷冻手术是一种用于治疗良性和恶性皮肤疾病的有效治疗技术。冷冻手术的主要作用机制与对治疗组织的血管效应有关。冷冻手术后，注射部位会形成过度增生的肉芽组织，这可能是冷冻剂血管生成刺激和炎症反应的结果，尤其是在内皮细胞中。
评估冷冻作为大鼠先前受伤皮肤愈合现象一部分的血管生成作用。
在20只大鼠的每只身上做两个切口，将它们随机分为两组，每组10只。3天后，对其中一个切口进行液氮冷冻手术。然后收集大鼠样本，进行切割和染色以进行组织病理学检查，评估不同时间和情况下的局部血管生成情况。
可以证明，冷冻手术尽管在应用后很快会促进细胞死亡并加剧局部炎症，但与未进行该手术的组织愈合相比，它能诱导受影响组织更快地细胞增殖，并在第二阶段维持该增殖速率。
这些发现，再加上单核细胞与新血管形成（在寒冷引起的损伤中形成丰富的新血管系统）之间存在直接关系这一认识，表明冷冻手术具有血管生成刺激作用，尽管完全愈合需要更长时间。统计检验的显著性水平为5%（p<0.05）。
