Vereshchagina Y V, Bulgakov V P, Grigorchuk V P, Rybin V G, Veremeichik G N, Tchernoded G K, Gorpenchenko T Y, Koren O G, Phan N H T, Minh N T, Chau L T, Zhuravlev Y N
Institute of Biology and Soil Science, Far Eastern Branch of the Russian Academy of Sciences, 159 Stoletija Str., Vladivostok, 690022, Russia.
Appl Microbiol Biotechnol. 2014 Sep;98(18):7773-80. doi: 10.1007/s00253-014-5869-2. Epub 2014 Jun 18.
Caffeoylquinic acids are found in artichokes, and they are currently considered important therapeutic or preventive agents for treating Alzheimer's disease and diabetes. We transformed artichoke [the cultivated cardoon or Cynara cardunculus var. altilis DC (Asteraceae)] with the rolC gene, which is a known inducer of secondary metabolism. High-performance liquid chromatography with UV and high-resolution mass spectrometry (HPLC-UV-HRMS) revealed that the predominant metabolites synthesized in the transgenic calli were 1,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, and chlorogenic acid. The rolC-transformed calli contained 1.5% caffeoylquinic acids by dry weight. The overall production of these metabolites was three times higher than that of the corresponding control calli. The enhancing effect of rolC remained stable over long-term cultivation.
咖啡酰奎宁酸存在于洋蓟中，目前被认为是治疗阿尔茨海默病和糖尿病的重要治疗或预防药物。我们用rolC基因转化了洋蓟[栽培的刺苞菜蓟或刺苞菜蓟变种Cynara cardunculus var. altilis DC（菊科）]，rolC基因是一种已知的次生代谢诱导剂。高效液相色谱结合紫外和高分辨率质谱（HPLC-UV-HRMS）分析表明，转基因愈伤组织中合成的主要代谢产物为1,5-二咖啡酰奎宁酸、3,4-二咖啡酰奎宁酸和绿原酸。经rolC转化的愈伤组织干重中含有1.5%的咖啡酰奎宁酸。这些代谢产物的总产量比相应的对照愈伤组织高3倍。rolC的增强作用在长期培养过程中保持稳定。
