Nezafati Mohammad Hassan, Nezafati Pouya, Amoueian Sakineh, Attaranzadeh Armin, Rahimi Hamid Reza
Student Research Committee, Mashhad University of Medical Sciences, No 124,, Niloufar 8th , Sadjad Blvd, Mashhad 91878, Iran.
J Cardiothorac Surg. 2014 Jun 17;9:101. doi: 10.1186/1749-8090-9-101.
The present study attempts to compare the immunohistochemistry (IHC) of von Willebrand factor (vWf) , endothelial cadherin, Caveolin and endothelial Nitric Oxide Synthase (eNOS) in VasoView Endoscopic Vein Harvesting (EVH) versus traditional Open Vein Harvesting (OVH) techniques for Coronary Artery Bypass Graft (CABG) Surgery performed in Javad al Aemeh Hospital of Mashhad, Iran in 2013,.
Forty-seven patients were scheduled for CABG (30 EVH and 17 OVH) among whom patients with relatively same gender and similar age were selected. Three separate two cm vein samples were harvested from each patient's saphenous vein. Each portion was collected from distal, middle and proximal zones of the saphenous vein. The tissues were deparaffinized, and antigen retrieval was done using EZ-retriever followed by an immunohistochemistry evaluation with vWf, e-cadherin, Caveolin and eNOS. In addition, demographic questioner as of Lipid profile, FBS, BMI, and cardiovascular risk factors were collected. Data analyses, including parametric and nonparametric tests were undertaken using the SPSS 16 software. A P value < 0.05 was regarded as statistically significant.
The mean age of the EVH and OVH groups were 63.76 ± 9.51 and 63.63 ± 8.31 years respectively with no significant difference between them (p = 0.989). In addition, there was no great difference between the EVH and OVH groups in lipid profile, DM, HTN, smoking history, CVA, and valvular dysfunction (P > 0.05). Qualitative report of vWf, e-cadherin, Caveolin and eNOS reveals no significant difference between the EVH and OVH (P > 0.05).
This study indicates that VasoView EVH technique causes no endothelial damage in comparison with OVH. This study could be a molecular confirmation for the innocuous of EVH technique.
本研究旨在比较2013年在伊朗马什哈德的贾瓦德·阿勒梅医院进行冠状动脉旁路移植术（CABG）时，VasoView内镜静脉采集（EVH）与传统开放静脉采集（OVH）技术中血管性血友病因子（vWf）、内皮钙黏蛋白、小窝蛋白和内皮型一氧化氮合酶（eNOS）的免疫组织化学（IHC）情况。
47例计划进行CABG的患者（30例EVH和17例OVH），从中选取性别相对相同、年龄相似的患者。从每位患者的大隐静脉采集3个单独的2厘米静脉样本。每个样本取自大隐静脉的远端、中段和近端区域。组织进行脱蜡处理，使用EZ-retriever进行抗原修复，随后用vWf、E-钙黏蛋白、小窝蛋白和eNOS进行免疫组织化学评估。此外，收集有关血脂谱、空腹血糖、体重指数和心血管危险因素的人口统计学调查问卷。使用SPSS 16软件进行数据分析，包括参数检验和非参数检验。P值<0.05被视为具有统计学意义。
EVH组和OVH组的平均年龄分别为63.76±9.51岁和63.63±8.31岁，两组之间无显著差异（p = 0.989）。此外，EVH组和OVH组在血脂谱、糖尿病（DM）、高血压（HTN）、吸烟史、脑血管意外（CVA）和瓣膜功能障碍方面也无显著差异（P>0.05）。vWf、E-钙黏蛋白、小窝蛋白和eNOS的定性报告显示，EVH组和OVH组之间无显著差异（P>0.05）。
本研究表明，与OVH相比，VasoView EVH技术不会造成内皮损伤。本研究可为EVH技术的无害性提供分子学证实。
