Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Nat Rev Mol Cell Biol. 2014 Jul;15(7):453-64. doi: 10.1038/nrm3821. Epub 2014 Jun 18.
During the lifetime of a cell proteins can change their localization, alter their abundance and undergo modifications, all of which cannot be assayed by tracking mRNAs alone. Methods to study proteomes directly are coming of age, thereby opening new perspectives on the role of post-translational regulation in stabilizing the cellular milieu. Proteomics has undergone a revolution, and novel technologies for the systematic analysis of proteins have emerged. These methods can expand our ability to acquire information from single proteins to proteomes, from static to dynamic measures and from the population level to the level of single cells. Such approaches promise that proteomes will soon be studied at a similar level of dynamic resolution as has been the norm for transcriptomes.
在细胞的生命周期中，蛋白质可以改变其定位、改变其丰度并发生修饰，所有这些都不能仅通过跟踪 mRNA 来检测。直接研究蛋白质组的方法已经成熟，从而为翻译后调控在稳定细胞环境中的作用开辟了新的视角。蛋白质组学发生了革命性的变化，出现了用于系统分析蛋白质的新技术。这些方法可以扩大我们从单个蛋白质到蛋白质组、从静态到动态测量以及从群体水平到单个细胞水平获取信息的能力。这些方法有望使蛋白质组学研究很快达到与转录组学相同的动态分辨率水平。
