Rutigliano Heloisa M, Adams Betty M, Jablonka-Shariff Albina, Boime Irving, Adams Thomas E
Department of Animal Science, University of California, Davis, California, USA.
Department of Animal Science, University of California, Davis, California, USA.
Theriogenology. 2014 Aug;82(3):455-60. doi: 10.1016/j.theriogenology.2014.05.010. Epub 2014 May 20.
The objective of this study was to determine the superovulatory potential of a single-chain analog of human FSH (Fcα) when administered to ewes either 3 days before, or coincident with, simulated luteolysis (pessary removal [PR]). A total of 40 animals were randomly assigned to receive Fcα at doses of 0.62, 1.25, or 2.5 IU/kg of body weight (bwt) 3 days before PR or 0.31, 0.62, 1.25, or 2.5 IU/kg of bwt at PR. Control ewes received protein without FSH activity. Blood samples were collected during the periovulatory period and ovarian tissue was collected 11 days after PR. Ovulation rate did not differ from the control group in ewes receiving the smallest doses of Fcα (0.31 and 0.62 IU/kg). However, a significant superovulatory response was noted in sheep receiving Fcα at doses of 1.25 and 2.5 IU/kg and this response was comparable in animals receiving the largest dose levels of Fcα at, or 3 days before, PR. The interval between PR and the LH surge was significantly extended and the LH surges were less synchronous in animals receiving Fcα at PR when compared with animals receiving the potent FSH agonist 3 days before PR. Taken together, these data indicate that the human single-chain gonadotropin with FSH activity promotes superovulation in ewe lambs in the breeding season. A single injection of the recombinant gonadotropin 3 days before luteolysis synchronizes the LH surge. The use of the single-chain analog of FSH in assisted reproduction for domestic animals is likely to be of practical significance as an alternative to conventional gonadotropins in superovulation protocols in livestock species.
本研究的目的是确定人促卵泡激素单链类似物（Fcα）在母羊模拟黄体溶解（取出阴道栓[PR]）前3天或同时给药时的超排潜力。总共40只动物被随机分配，在PR前3天接受剂量为0.62、1.25或2.5 IU/kg体重（bwt）的Fcα，或在PR时接受剂量为0.31、0.62、1.25或2.5 IU/kg bwt的Fcα。对照母羊接受无促卵泡激素活性的蛋白质。在排卵期采集血样，并在PR后11天采集卵巢组织。接受最小剂量Fcα（0.31和0.62 IU/kg）的母羊排卵率与对照组无差异。然而，接受1.25和2.5 IU/kg剂量Fcα的绵羊出现了显著的超排反应，并且在PR时或PR前3天接受最大剂量水平Fcα的动物中，这种反应相当。与在PR前3天接受强效促卵泡激素激动剂的动物相比，在PR时接受Fcα的动物中，PR与促黄体生成素峰之间的间隔显著延长，促黄体生成素峰的同步性较差。综上所述，这些数据表明具有促卵泡激素活性的人单链促性腺激素可促进繁殖季节母羊超排。在黄体溶解前3天单次注射重组促性腺激素可使促黄体生成素峰同步。在家畜辅助生殖中使用促卵泡激素单链类似物作为家畜超排方案中传统促性腺激素的替代品可能具有实际意义。
