Li Nan, Han Zhenzhen, Li Lin, Zhang Bing, Liu Zhidong, Li Jiawei
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, People's Republic of China.
Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, People's Republic of China.
Drug Des Devel Ther. 2018 May 23;12:1399-1411. doi: 10.2147/DDDT.S160524. eCollection 2018.
The objective of this study was to investigate the effects of the solid lipid nanoparticles of baicalin (BA-SLNs) on an experimental cataract model and explore the molecular mechanism combined with bioinformatics analysis.
The transparency of lens was observed daily by slit-lamp and photography. Lenticular opacity was graded. Two-dimensional gel electrophoresis (2-DE) was employed to analyze the differential protein expression modes in each group. Proteins of interest were subjected to protein identification by nano-liquid chromatography tandem mass spectrometry (LC-MS/MS). Bioinformatics analysis was performed using the Ingenuity Pathway Analysis (IPA) online software to comprehend the biological implications of the proteins identified by proteomics.
At the end of the sodium selenite-induced cataract progression, almost all lenses from the model group developed partial nuclear opacity; however, all lenses were clear and normal in the blank group. There was no significant difference between the BA-SLNs group and the blank group. Many protein spots were differently expressed in 2-DE patterns of total proteins of lenses from each group, and 65 highly different protein spots were selected to be identified between the BA-SLNs group and the model group. A total of 23 proteins were identified, and 12 of which were crystalline proteins.
We considered crystalline proteins to play important roles in preserving the normal expression levels of proteins and the transparency of lenses. The general trend in the BA-SLN-treated lenses' data showed that BA-SLNs regulated the protein expression mode of cataract lenses to normal lenses. Our findings suggest that BA-SLNs may be a potential therapeutic agent in treating cataract by regulating protein expression and may also be a strong candidate for future clinical research.
本研究旨在探讨黄芩苷固体脂质纳米粒（BA-SLNs）对实验性白内障模型的影响，并结合生物信息学分析探索其分子机制。
每天用裂隙灯和摄影观察晶状体透明度。对晶状体混浊进行分级。采用二维凝胶电泳（2-DE）分析各组差异蛋白表达模式。通过纳升液相色谱串联质谱（LC-MS/MS）对感兴趣的蛋白质进行鉴定。使用 Ingenuity Pathway Analysis（IPA）在线软件进行生物信息学分析，以了解蛋白质组学鉴定的蛋白质的生物学意义。
在亚硒酸钠诱导的白内障进展末期，模型组几乎所有晶状体均出现部分核混浊；然而，空白组所有晶状体均清晰正常。BA-SLNs组与空白组之间无显著差异。各组晶状体总蛋白的2-DE图谱中有许多蛋白点表达不同，在BA-SLNs组和模型组之间选择了65个差异较大的蛋白点进行鉴定。共鉴定出23种蛋白质，其中12种为晶状体蛋白。
我们认为晶状体蛋白在维持蛋白质正常表达水平和晶状体透明度方面起重要作用。BA-SLNs处理的晶状体数据的总体趋势表明，BA-SLNs将白内障晶状体的蛋白质表达模式调节为正常晶状体。我们的研究结果表明，BA-SLNs可能是一种通过调节蛋白质表达治疗白内障的潜在治疗剂，也可能是未来临床研究的有力候选药物。
