Silva Ana Rita Marques da, Pinto Shelon Cristina Souza, Santos Elizabete Brasil dos, Santos Fábio André dos, Farago Paulo Vitor, Gomes João Carlos, Pina-Vaz Irene, Carvalho Manuel Fontes
PhD Student, Department of Endodontics, School of Dentistry, University of Porto, Porto, Portugal.
PhD, State University of Ponta Grossa, Postgraduate Program in Dentistry, CEP 84030-900, Uvaranas, Ponta Grossa Paraná Brazil.
J Contemp Dent Pract. 2014 Jan 1;15(1):61-5. doi: 10.5005/jp-journals-10024-1188.
The present study aims to evaluate the antimicrobial effect of two new intracanal preparations against E. faecalis. Thirty single-rooted human canine teeth were used. The crowns were removed and the roots were instrumented using a conventional technique. Three groups of ten teeth each were infected with 108 CFU/ ml of E. faecalis for 21 days. The root canals were flled with new intracanal medications containing 3% doxycycline hydrochloride (DX) or 2% chlorhexidine digluconate (CHX). Ten teeth received no medication (NM)-negative control. Microbial samples were obtained 21 days after contamination: 14 days under the effect of the intracanal medications and 7 days after replacing the medications by BHI broth. The samples were homogenized, diluted, seeded on BHI agar and incubated for 48h/36°C. The number of colony forming units (CFU/ml) was obtained and analyzed statistically. All intracanal dressings significantly reduced the number of bacterial cells in the root canal after 14 days with medication. After the period with 7 days with BHI broth, the CFU counts of E. faecalis remained at low values. However, the NM group showed a significant increase of CFU in this period to similar values of the initial contamination. 3% doxycycline hydrochloride gel and 2% CHX gel were effective to eliminate E. faecalis from the root canal system.
本研究旨在评估两种新型根管内制剂对粪肠球菌的抗菌效果。使用了30颗单根人犬齿。去除牙冠并采用传统技术对牙根进行预备。将三组，每组十颗牙齿，感染浓度为108 CFU/ml的粪肠球菌，持续21天。根管内填充含有3%盐酸多西环素（DX）或2%葡萄糖酸氯己定（CHX）的新型根管内药物。十颗牙齿未用药（NM）作为阴性对照。在污染21天后获取微生物样本：在根管内药物作用14天以及用脑心浸液肉汤替换药物7天后。将样本匀浆、稀释，接种于脑心浸液琼脂上，并在36°C孵育48小时。获得菌落形成单位（CFU/ml）数量并进行统计学分析。所有根管内敷料在用药14天后均显著减少了根管内细菌细胞数量。在用脑心浸液肉汤处理7天后，粪肠球菌的CFU计数保持在较低水平。然而，NM组在此期间CFU显著增加至与初始污染相似的值。3%盐酸多西环素凝胶和2% CHX凝胶可有效从根管系统中清除粪肠球菌。
